Abstract
A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Antigens, Bacterial / genetics*
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Bacterial Capsules / genetics
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Bacterial Typing Techniques / methods*
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Child
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Child, Preschool
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Genotype
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Humans
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Infant
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Mozambique / epidemiology
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Pneumococcal Infections / epidemiology
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Pneumococcal Infections / microbiology*
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Polymerase Chain Reaction / methods*
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Serotyping / methods
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Streptococcus pneumoniae / classification*
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Streptococcus pneumoniae / genetics*
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Streptococcus pneumoniae / isolation & purification