Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

Iyo Takemura-Uchiyama; Jumpei Uchiyama; Makoto Osanai; Norihito Morimoto; Tadashi Asagiri; Takako Ujihara; Masanori Daibata; Tetsuro Sugiura; Shigenobu Matsuzaki

doi:10.1016/j.micinf.2014.02.011

Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

Microbes Infect. 2014 Jun;16(6):512-7. doi: 10.1016/j.micinf.2014.02.011. Epub 2014 Mar 12.

Authors

Iyo Takemura-Uchiyama¹, Jumpei Uchiyama², Makoto Osanai³, Norihito Morimoto⁴, Tadashi Asagiri⁴, Takako Ujihara⁵, Masanori Daibata², Tetsuro Sugiura⁴, Shigenobu Matsuzaki⁶

Affiliations

¹ Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
² Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Kochi University, Kochi, Japan.
³ Department of Pathology, Faculty of Medicine, Kochi University, Kochi, Japan.
⁴ Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
⁵ Science Research Center, Kochi University, Kochi, Japan.
⁶ Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Kochi University, Kochi, Japan. Electronic address: matuzaki@kochi-u.ac.jp.

PMID: 24631574
DOI: 10.1016/j.micinf.2014.02.011

Abstract

Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6 h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia in vitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.

Keywords: Bacteriophage; Mouse; Podoviridae; Therapeutic; Therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Therapy*
Cross Infection / microbiology
Cross Infection / therapy
Disease Models, Animal
Lung / microbiology
Lung / pathology
Methicillin-Resistant Staphylococcus aureus / virology*
Mice
Sepsis / therapy*
Staphylococcal Infections / therapy*
Staphylococcus Phages*