Neutrophil-to-lymphocyte ratio is effective prognostic indicator for post-amputation patients with critical limb ischemia ========================================================================================================================= * Qi Wang * Han Liu * Siqiao Sun * Zhihua Cheng * Yang Zhang * Xiwei Sun * Zhongying Wang * Shuai Wang ## Abstract **Objectives:** To confirm whether neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are indicators for the prognosis of post-amputation patients with critical limb ischemia (CLI). **Methods:** In this retrospective observational study a total 270 post-amputation patients with CLI were included between January 2010 and December 2014 in the First Hospital of Jilin University, Changchun, China. The neutrophil and lymphocyte counts were recorded before amputations. Neutrophil-to-lymphocyte ratio was calculated and NLR ≥8.08 was defined as elevated. Logistic regression analysis was conducted to test the prognostic value. **Results:** According to the statistical analysis, it was indicated that NLR ≥8.08 (odds ratio [OR] 26.228, 95% confidence interval [CI]: 5.801-118.583, *p*<0.001), PLR ≥237.14 (OR: 3.464, 95% CI: 1.289-9.308, *p*=0.014) and coronary heart disease (OR: 2.739, 95% CI: 1.060-7.082, *p*=0.038) were the independent prognostic indicators for the patients. **Conclusion:** Neutrophil-to-lymphocyte ratio, PLR, and coronary heart disease are independent prognostic indicators for post-amputation patients with CLI. Critical limb ischemia (CLI) is usually caused by a severe blockage, such as arteriosclerotic plaque and thrombus, and so forth in the arteries of the lower extremities that markedly reduces blood flow. The incidence of CLI is 0.35% of the eligible study population in the United States.1 With a low limb salvage rate and rapid progress, the patients always suffer from a series of ischemic symptoms, such as intermittent claudication, rest pain, and so forth. The pathological terminations are ulcer and gangrene of limb. Once the disease is diagnosed, the systemic treatments, such as bypass surgery and endovascular intervention therapy should be conducted.2,3 However, there are still 30-50% of the patients with CLI who have to suffer from amputation instead of revascularization due to some reasons.4 Prognosis for post-amputation patients, especially with diabetes mellitus, are usually poor.5,6 According to a domestic study, for post-amputation patients with CLI and diabetes mellitus, 1-year mortality range from 13% to 40% and 5-year mortality range from 39% to 80%, which are higher than some patients with tumor.7 In recent years, neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been demonstrated to be indicators in systemic inflammatory response (SIR), and SIR has a close connection with prognosis of patients with tumor.8,9 Similarly, limb ischemia and gangrene can lead to SIR, whose pathological characteristics are inflammatory cell infiltration, metabolite accumulating and tissue necrosis, and so forth. As a trauma stress, amputation can promote SIR as well. However, there is still no related research on the relation between NLR/PLR and the prognosis of post-amputation patients with CLI. We conducted this study to confirm whether NLR and PLR are indicators for the prognosis of post-amputation patients with CLI. ## Methods *Selection criteria*. All of the patients suffered from chronic limb ischemia (>3 weeks) or acute limb ischemia (≤3 weeks) with severe limb ulcer or gangrene (Rutherford category V or VI), and a series of routine examinations, such as blood routine, serum lipid, and protein examination were performed before amputation. ### Exclusion criteria Patients with the disease which can lead to the abnormal white blood cell and platelet count, including inflammatory diseases (such as, pulmonary infection and biliary tract infection), leukemia, tumor, severe renal or hepatic dysfunction, abnormal thyroid functions, metabolic syndrome, bone marrow or hematologic disorders, splenectomy, thrombotic thrombocytopenic purpura, idiopathic thrombocytopenic purpura, myeloproliferative disorders, and radiation. Two-hundred and seventy patients complying with the previously mentioned criteria were retrospectively recruited from January 2010-December 2014 in The First Hospital of Jilin University, Changchun, China. These patients included 187 males and 83 females (male/female ratio=2.25:1), aged between 60 and 88 years (mean age: 71±6 years). According to prognosis of the patients, they were divided into 2 groups: poor prognosis group (including myocardial infarction, stroke, and death cases within 30 days) and contrast group. The study protocol, according to principles of Helsinki Declaration, was reviewed and approved by the Ethics Committee of The First Hospital of Jilin University. Patients with acute ischemia (77 cases), usually underwent sudden pallor, pain, and paralysis of limbs. Due to lack of timely and prompt treatments, most of them had suffered from limb gangrene before hospitalization. In spite of revascularization, others also underwent amputation due to irreversible damage or extreme ischemia in this study. Patients with chronic ischemia (193 cases), always underwent long-term intermittent claudication or rest pain, eventually limb ulcer and gangrene. There are 30 cases for minor amputation (toe and foot amputation) and 240 cases for major amputation (above and below knee amputation). Medicine treatments, including vascular dilation and anti-infection, were performed for the patients after amputation. Within 30 days after amputation, there were 9 cases with myocardial infarction or stroke, and 22 cases of death. All test data of the patients were collected using the Hospital Information System (HISystem V3.0, Zhejiang MediCARE I.T.CO., LTD). We extracted the last routine examination data before amputation. In this study, NLR is the ratio of absolute neutrophil count and PLR is the ratio of absolute platelet count divided by the absolute lymphocyte count. ### Statistical analysis Data were presented as mean±standard deviation (SD) or the absolute number of subjects. The Q-Q plots and the Shapiro-Wilk test were examined to assess data normality. Levene’s test was performed for variance homogeneity and Kruskal-Wallis test was applied when analysis of variance was not applicable. Chi-squared or Fisher’s exact test was used for comparing the differences between the groups. Receiver operating characteristic (ROC) curves were performed to identify the indicative performances of NLR and PLR in post-amputation patients with critical limb ischemia. The areas under the curves (AUC) were calculated with 95% confidence intervals (CI). The Youden index was performed to determine optimal cut-off values for each indicator. Logistic regression model was conducted to verify prognostic factors. Sensitivity, specificity, positive, and negative predictive values. Positive and negative likelihood ratios were calculated with 95% CIs. The statistical analysis was performed using the Statistical Package for Social Sciences version 19 software (IBM Corp., Armonk, NY, USA). In all analysis, *p*<0.05 was considered statistically significant. ## Results In this study, the data of blood examination and the history of patients, such as smoking history, hypertension (blood pressure >140/90 mm Hg), diabetes mellitus (fasting blood glucose >7.0 mmol/L or 2 hours postprandial blood glucose >11.1 mmol/L), coronary heart disease, hyperlipidemia (total cholesterol >5.72 mmol/L, or triglyceride >1.70 mmol/L) and cerebral apoplexy were identified according to the electronic medical records (**Table 1**). View this table: [Table 1](http://smj.org.sa/content/38/1/24/T1) Table 1 General characteristics and history of 270 patients with critical limb ischemia. As shown in **Table 1**, we observed that the coronary heart disease (*p*=0.002), NLR (*p*<0.001), PLR (*p*<0.001), and albumin (*p*=0.004) were statistically significant. According to **Figure 1** and **Table 2**, NLR ≥8.08 was primary hazard factor with odds ratio [OR]: 26.228. Sensitivity and specificity of NLR were greater than the others. In **Figure 2**, it was shown that NLR, PLR, and albumin were linearly associated with each other. ![Figure 1](http://smj.org.sa/https://smj.org.sa/content/smj/38/1/24/F1.medium.gif) [Figure 1](http://smj.org.sa/content/38/1/24/F1) Figure 1 The receiver operating characteristic (ROC) curves for A) neutrophil to lymphocyte ratio (NLR) (the area under the curve (AUC) is 0.898 and the cut-off value is 8.08, with a sensitivity of 0.935 and a specificity of 0.757 for NLR), B) platelet to lymphocyte ratio (PLR) (the AUC is 0.727 and the cut-off value is 237.14, with a sensitivity of 0.774 and a specificity of 0.644 for PLR), and C) albumin in amputated patients with critical limb ischemia (the AUC is 0.659 and the cut-off value is 25.35, with a sensitivity of 0.828 and a specificity of 0.484 for albumin). View this table: [Table 2](http://smj.org.sa/content/38/1/24/T2) Table 2 Multi-variate Logistic regression of 270 patients with critical limb ischemia. ![Figure 2](http://smj.org.sa/https://smj.org.sa/content/smj/38/1/24/F2.medium.gif) [Figure 2](http://smj.org.sa/content/38/1/24/F2) Figure 2 Correlation among neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and albumin for patients in 2 groups. A) There was a positive correlation between NLR and PLR, R=0.618, *p*<0.001. B) There was a negative correlation between NLR and albumin, R=-0.233, *p*<0.001. C) There was a negative correlation between PLR and albumin, R=-0.238, *p*<0.001. ## Discussion Critical limb ischemia is a severe arteriosclerotic plaque or thrombus blockage in the arteries of the lower extremities that markedly reduces blood flow, whose major symptoms are intermittent claudication, rest pain, limb ulcer, and gangrene. Amputation is inevitable once a limb gangrene appear. The randomized, controlled trials, which served as basis for the New England Society for Vascular Surgery (SVS) safety and efficacy objective performance goals (OPGs) showed that mortality and major amputation rates at 30 days were 2.8% and 2% compared with 2.7% and 1.9% for the patients with CLI.10-12 A close connection between mortality and infectious complications (such as, sepsis, pneumonia and urinary tract infection, and so forth) has been confirmed by Curran et al’s study.13 For patients with CLI, limb gangrene always comes with tissue necrosis, systemic infection, and inflammation, which can increase mortality. Even worse, harmful metabolites and pain stress lead to poor prognosis as well. As a result, some of them might be very weak to undergo amputation, which causes traumatic stress to the body. At the same time, NLR had been confirmed as the indicator for inflammation in circulatory, respiratory, digestive, urinary disease, and solid tumor.14-17 Also, PLR had been demonstrated to have a close connection with prognosis of patients with tumor.18,19 Similarly, NLR and PLR could indicate the inflammation, which is induced by limb gangrene and demonstrate the risk level for the patient with CLI to suffer from amputation. Therefore, NLR and PLR might be the prognostic indicators for post-amputation patient with CLI. According to the ROC curves, the values of AUC NLR was 0.898 and PLR was 0.727. This means that, especially NLR have highly clinical significance. As shown in **Table 2**, the OR values of NLR ≥8.08 and PLR ≥237.14 were 26.228 and 3.464 (*p*<0.005). This meant that the patients with NLR ≥8.08 or PLR ≥237.14, were controlling the other factors, are more likely (26.228 times and 3.464 times than the other patients) to undergo the worse prognosis. Moreover, the combination of NLR and PLR had been verified more indicative in prognosis by the Logistic regression analysis. In recent years, some studies20-23 on the relationship between NLR and prognosis of patients with non-small cell lung cancer concluded the cut-off values between 2.093 to 5.0. In Wu’s study,24 the cut-off value of NLR for patients with non-small cell lung cancer was 2.68, which was much less than the value in this study. Acute inflammation is almost simultaneous with a sharper increasing count of white blood cell and neutrophil cell than chronic inflammation. Critical limb ischemia often combines with limb gangrene, which can lead to limb infection and acute inflammation. Based on the oncothlipsis effect of tumor and other reasons, patients with non-small cell lung cancer always suffer from obstruction or compression of airway and intrapulmonary infection, which always lead to chronic inflammation. Therefore, the cut-off value of NLR in this study is greater than in other studies, and it is more indicative for systemic acute inflammation. Moreover, the connection between NLR and amputation has been confirmed by other studies as well.25,26 Luo’s study27 showed that patients with a post-treatment NLR ≥3.8 are likely to suffer from amputation. It is still less than the value in the present study. With NLR ≥3.8, the patient takes higher risk to suffer from amputation. With NLR ≥8.08, the post-amputation patient is likely to receive a worse prognosis. Actually, the results are not contrary to the studies. Besides, coronary heart disease was another prognostic factor (OR: 2.739, 95%CI: 1.060-7.082, *p*=0.038). Arteriosclerosis is a systemic disease. Coronary arteriosclerosis merely is a local manifestation of systemic arteriosclerosis. Patients with coronary heart disease always undergo arteriosclerosis of limb arteries, mesenteric arteries, renal arteries, and carotid arteries, which could restrain the tolerance of relevant organs.28 It might be one reason of the worse prognosis. In our study, we pointed out that NLR and PLR are the indicators for the prognosis of the post-amputation patients with CLI for the first time. The results are significant for the clinical treatment and evaluation of the patients with CLI. However, as a single-center and retrospective study, there are some limitations. Additional larger sample, multi-center, and prospective studies are necessary in future studies. In conclusion, NLR, PLR, and coronary heart disease are independent prognostic indicators for post-amputation patients with CLI. With NLR ≥8.08, PLR ≥237.14 and coronary heart disease, the patients are more likely to suffer from poor prognosis (including myocardial infarction, stroke, and death cases within 30 days) than patients without those risk factors. Especially NLR ≥8.08, which is a primary risk factor for the patients (OR: 26.228, 95%CI: 5.801-118.583, *p*<0.001). ## Acknowledgment *Authors gratefully acknowledge Dr. Sean Luo, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA, and Medjaden Bioscience Co., Ltd. Hongkong, China for the assistance of medical editing and writing*. ## Footnotes * **Disclosure.** Authors have no conflict of interest, and the work was not supported or funded by any drug company. * Received July 25, 2016. * Accepted October 20, 2016. * Copyright: © Saudi Medical Journal This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ## References 1. Nehler MR, Duval S, Diao L, Annex BH, Hiatt WR, Rogers K, et al. (2014) Epidemiology of peripheral arterial disease and critical limb ischemia in an insured national population. J Vasc Surg 60:686–695. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.jvs.2014.03.290&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24820900&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 2. Miyahara T, Suhara M, Nemoto Y, Shirasu T, Haga M, Mochizuki Y, et al. (2015) Long-term results of treatment for critical limb ischemia. Ann Vasc Dis 8:192–197. 3. Chang SH, Tsai YJ, Chou HH, Wu TY, Hsieh CA, Cheng ST, et al. (2014) Clinical predictors of long-term outcomes in patients with critical limb ischemia who have undergone endovascular therapy. Angiology 65:315–322. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1177/0003319713515544&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24357434&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 4. Hankey GJ, Norman PE, Eikelboom JW (2006) Medical treatment of peripheral arterial disease. JAMA 295:547–553. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1001/jama.295.5.547&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=16449620&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) [Web of Science](http://smj.org.sa/lookup/external-ref?access_num=000234990300022&link_type=ISI) 5. Lo RC, Bensley RP, Dahlberg SE, Matyal R, Hamdan AD, Wyers M, et al. (2014) Presentation, treatment, and outcome differences between men and women undergoing revascularization or amputation for lower extremity peripheral arterial disease. J Vasc Surg 59:409–418. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.jvs.2013.07.114&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24080134&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 6. Won SH, Chung CY, Park MS, Lee T, Sung KH, Lee SY, et al. (2014) Risk factors associated with amputation-free survival in patient with diabetic foot ulcers. Yonsei Med J 55:1373–1378. 7. Fei YF, Wang C, Chen DW, Li YH, Lin S, Liu GJ, et al. (2012) Incidence and risk factors of amputation among inpatients with diabetic foot. Natl Med J China 92:1686–1689. 8. Templeton AJ, McNamara MG, Seruga B, Vera-Badillo FE, Aneja P, Ocana A, et al. (2014) Prognostic role of neutrophil-to-lymphocyte ratio in solid tumors: a systematic review and meta-analysis. J Natl Cancer Inst 106:dju124. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1093/jnci/dju124&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24875653&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 9. Zhou X, Du Y, Huang Z, Xu J, Qiu T, Wang J, et al. (2014) Prognostic value of PLR in various cancers: a meta-analysis. PLoS One 9:e101119. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1371/journal.pone.0101119&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24968121&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 10. Conte MS, Geraghty PJ, Bradbury AW, Hevelone ND, Lipsitz SR, Moneta GL, et al. (2009) Suggested objective performance goals and clinical trial design for evaluating catheter-based treatment of critical limb ischemia. J Vasc Surg 50:1462–1473. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.jvs.2009.09.044&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=19897335&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 11. Goodney PP, Schanzer A, Demartino RR, Nolan BW, Hevelone ND, Conte MS, et al. (2011) Vascular Study Group of New England. Validation of the Society for Vascular Surgery’s objective performance goals for critical limb ischemia in everyday vascular surgery practice. J Vasc Surg 54:100–108. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.jvs.2010.11.107&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=21334173&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 12. Vierthaler L, Callas PW, Goodney PP, Schanzer A, Patel VI, Cronenwett J, et al. (2015) Determinants of survival and major amputation after peripheral endovascular intervention for critical limb ischemia. J Vasc Surg 62:655–664. 13. Curran T, Zhang JQ, Lo RC, Fokkema M, McCallum JC, Buck DB, et al. (2014) Risk factors and indications for readmission after lower extremity amputation in the American College of Surgeons National Surgical Quality Improvement Program. J Vasc Surg 60:1315–1324. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.jvs.2014.05.050&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24985536&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 14. Nunez J, Nunez E, Bodi V, Sanchis J, Minana G, Mainar L, et al. (2008) Usefulness of the neutrophil to lymphocyte ratio in predicting long-term mortality in ST segment elevation myocardial infarction. Am J Cardiol 101:747–752. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.amjcard.2007.11.004&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=18328833&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) [Web of Science](http://smj.org.sa/lookup/external-ref?access_num=000254024000001&link_type=ISI) 15. Kishi Y, Kopetz S, Chun YS, Palavecino M, Abdalla EK, Vauthey JN (2009) Blood neutrophil-to-lymphocyte ratio predicts survival in patients with colorectal liver metastases treated with systemic chemotherapy. Ann Surg Oncol 16:614–622. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1245/s10434-008-0267-6&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=19130139&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) [Web of Science](http://smj.org.sa/lookup/external-ref?access_num=000263351700012&link_type=ISI) 16. Shimada H, Takiguchi N, Kainuma O, Soda H, Ikeda A, Cho A, et al. (2010) High preoperative neutrophil-lymphocyte ratio predicts poor survival in patients with gastric cancer. Gastric Cancer 13:170–176. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1007/s10120-010-0554-3&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=20820986&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) [Web of Science](http://smj.org.sa/lookup/external-ref?access_num=000284961300005&link_type=ISI) 17. Gunay E, Sarınc Ulasli S, Akar O, Ahsen A, Gunay S, Koyuncu T, et al. (2014) Neutrophil-to-lymphocyte ratio in chronic obstructive pulmonary disease: a retrospective study. Inflammation 37:374–380. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1007/s10753-013-9749-1&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24078279&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 18. Pinato DJ, Mauri FA, Ramakrishnan R, Wahab L, Lloyd T, Sharma R (2012) Inflammation-based prognostic indices in malignant pleural mesothelioma. J Thorac Oncol 7:587–594. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1097/JTO.0b013e31823f45c1&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=22307011&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 19. Wang DS, Luo HY, Qiu MZ, Wang ZQ, Zhang DS, Wang FH, et al. (2012) Comparison of the prognostic values of various inflammation based factors in patients with pancreatic cancer. Med Oncol 29:3092–3100. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1007/s12032-012-0226-8&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=22476808&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 20. Pinato DJ, Shiner RJ, Seckl MJ, Stebbing J, Sharma R, Mauri FA (2014) Prognostic performance of inflammation-based prognostic indices in primary operable non-small cell lung cancer. Br J Cancer 110:1930–1935. 21. Teramukai S, Kitano T, Kishida Y, Kawahara M, Kubota K, Komuta K, et al. (2009) Pretreatment neutrophil count as an independent prognostic factor in advanced non-small-cell lung cancer: an analysis of Japan Multinational Trial Organisation LC00-03. Eur J Cancer 45:1950–1958. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.ejca.2009.01.023&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=19231158&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) [Web of Science](http://smj.org.sa/lookup/external-ref?access_num=000268472700008&link_type=ISI) 22. Jafri SH, Shi R, Mills G (2013) Advance lung cancer inflammation index (ALI) at diagnosis is a prognostic marker in patients with metastatic non-small cell lung cancer (NSCLC): a retrospective review. BMC Cancer 13:158. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1186/1471-2407-13-158&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=http://www.n&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 23. Lee Y, Kim SH, Han JY, Kim HT, Yun T, Lee JS (2012) Early neutrophil-to-lymphocyte ratio reduction as a surrogate marker of prognosis in never smokers with advanced lung adenocarcinoma receiving gefitinib or standard chemotherapy as first-line therapy. J Cancer Res Clin Oncol 138:2009–2016. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1007/s00432-012-1281-4&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=22772951&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 24. Wu G, Yao Y, Bai C, Zeng J, Shi D, Gu X, et al. (2015) Combination of platelet to lymphocyte ratio and neutrophil to lymphocyte ratio is a useful prognostic factor in advanced non-small cell lung cancer patients. Thorac Cancer 6:275–287. 25. Gonzalez-Fajardo JA, Brizuela-Sanz JA, Aguirre-Gervas B, Merino-Diaz B, Del Rio-Sola L, Martin-Pedrosa M, et al. (2014) Prognostic significance of an elevated neutrophil-lymphocyte ratio in the amputation-free survival of patients withchronic critical limb ischemia. Ann Vasc Surg 28:999–1004. 26. Tasoglu I, Cicek OF, Lafci G, Kadirogullari E, Sert DE, Demir A, et al. (2014) Usefulness of neutrophil/lymphocyte ratio as a predictor of amputation after embolectomy for acute limb ischemia. Ann Vasc Surg 28:606–613. [CrossRef](http://smj.org.sa/lookup/external-ref?access_num=10.1016/j.avsg.2012.12.009&link_type=DOI) [PubMed](http://smj.org.sa/lookup/external-ref?access_num=24084272&link_type=MED&atom=%2Fsmj%2F38%2F1%2F24.atom) 27. Luo H, Yuan D, Yang H, Yukui M, Huang B, Yang Y, et al. (2015) Post-treatment neutrophil-lymphocyte ratio independently predicts amputation in critical limb ischemia without operation. Clinics 70:273–277. 28. Zheng LQ, Yu JM, Li J, Sun ZQ, Sun YX, Hu DY (2007) Mean level of ankle-brachial index and prevalence of peripheral arterial disease among inpatients with equal-risk to coronary heart disease. Chin J Arterioscler 15:857–860.