Evaluation of irisin, retinol-binding protein 4, and leptin serum levels as biomarkers of macrovascular complications involvement in Saudi type 2 diabetes mellitus. A case-control study ========================================================================================================================================================================================= * Ehdaa Y. Ali * Gehan A. Hegazy * Enayat M. Hashem ## Abstract **Objectives:** To evaluate adipokine serum values of irisin, retinol-binding protein 4, and leptin in Saudi cases with type 2 diabetes mellitus (T2DM) for providing markers of T2DM macrovascular complications. **Methods:** This case-control research was carried out at Erfan Hospital, Jeddah, Saudi Arabia. The study included 138 subjects, classified into 3 groups: 46 T2DM patients with macrovascular complications, 46 T2DM without macrovascular complications, and 46 controls. Participants evaluated clinically and some biochemical parameters were measured. **Results:** Diabetic with and without macrovascular complications showed elevation of retinol-binding protein 4 (RBP4) and leptin; decreased irisin serum levels versus controls. Serum irisin was lower (*p*=0.007), while RBP4 was higher (p<0.0001) in T2DM patients with macrovascular complications versus without. Irisin showed negative correlations with fasting blood glucose (FBG), insulin, homeostatic model assessment of insulin resistance (HOMA-IR), RBP4, hemoglobin A1C (HbA1C), triglyceride, cholesterol, and low-density lipoprotein cholesterol. While RBP4 showed positive correlations with fasting blood glucose, insulin, HOMA-IR, leptin, and HbA1c; but a negative association with high-density lipoprotein cholesterol. **Conclusion:** Type 2 DM patients had raised RBP4 and leptin, but lower irisin levels versus controls. Irisin was lower, but RBP4 was higher in T2DM patients with macrovascular complications versus without, suggesting T2DM patients in pro-inflammatory conditions. These results suggested that irisin is protective, while RBP4 is a risk factor for T2DM macrovascular complications. * irisin * leptin * retinol-binding protein 4 * macrovascular complications * type 2 diabetes mellitus Diabetes mellitus (DM) is a metabolic defect caused by impaired insulin action, secretion, or both. Approximately 451 million people globally diabetic, it is predicted that by 2040 approximately 693 million people (9.9%) will have type 2 diabetes mellitus (T2DM) constituents 85-90% of diabetes cases.1 Kingdom of Saudi Arabia (KSA) has the second-highest rate of T2DM prevalence in the Middle East.2 The inflammatory process is one of the T2DM pathophysiological mechanisms. Throughout glycemic control deterioration, various factors affect the endothelium leading to endothelial dysfunction that deteriorates with insulin resistance (IR); these conditions are associated with T2DM macrovascular complications (MVC). Cardiovascular diseases (CVD) risk increased 2-fold to 4-fold in T2DM patients.3 Adipose tissue acts as an endocrine organ through inflammatory adipokines secretion that participates in IR in various tissues. Irisin is myokine that is proteolytically separated from transmembrane fibronectin type III domain-containing protein 5 (FNDC5). It has beneficial actions on metabolism. Irisin is inversely associated with inflammation that leads to IR.4 Adipocytokine retinol-binding protein 4 (RBP4) is a 21-kDa protein of the lipocalin superfamily. It is only circulatory retinol (vitamin A) carrier protein and encoded by the RBP4 gene, localized in 10q23-q24 chromosome.5 Researchers reported that RBP4 was related to obesity-associated with metabolic defects, and serum RBP4 positively associated with subclinical carotid intima-media thickness and atherosclerosis in T2DM.6 Leptin is an adipokine hormone encoded by the ob gene. It has a role in adiposity and thermogenesis. Leptin and its receptor isoforms are expressed in adipose tissue and cardiovascular tissues as cells of smooth muscles, endothelial cells, and cardiomyocytes. Hyperleptinemia is associated with obesity, T2DM, and IR. Leptin peripheral effects enhanced inflammatory reactions, atherogenesis, oxidative stress, and thrombosis, leading to arterial wall stiffness, endothelial defects, and atherosclerotic plaques development.7 This study aimed to search for adipokines levels as irisin, RBP4, and leptin in T2DM patients and their association with MVC to provide reasonable MVC new markers in T2DM. ## Methods This case-control study was conducted at Erfan Hospital, Jeddah, Saudi Arabia, between January 2018-2019. The research was confirmed by the Biomedical Ethics Research Committee at King Abdulaziz University (Approval #62-15) that was formed according to the Declaration of Helsinki. All patients were randomly chosen from outpatient endocrinology and cardiology clinics at Erfan Hospital. All participants signed informed consent to participate in the research. Excluded from the research were patients with T1DM, inflammatory diseases as bronchial asthma, gout, rheumatoid arthritis, ulcerative colitis, hepatitis, thyroiditis, nephritis, multiple sclerosis, allergic reactions, Crohn’s disease, lupus erythematosus, congenital CVD or CVD caused by trauma, CVD diagnosed before T2DM, or cancer treated by chemotherapy or chronic kidney diseases. The sample size was estimated before the study using the Raosoft software package for sample size calculation. By assuming that 50% of patients will have the disease, the required sample size was 92 patients at a 5% error margin and a 95% confidence interval (CI). A total of 138 subjects of both genders between 40 and 80 years old were included. Subjects were categorized into 46 T2DM patients without MVC, 46 T2DM patients with MVC, and 46 healthy subjects that matched patients in age, gender, and body mass index (BMI) served as control. Type 2 DM diagnosis was based upon the American Diabetic Association’s (ADA) criteria of fasting blood glucose level ≥7.0 mmol/L. Macrovascular complications was defined based on the patient’s medical profile and diagnosed based upon American Heart Association criteria (AHA). Macrovascular complications included coronary artery diseases (CAD) established by coronary angiography, peripheral vascular diseases (PVD) diagnosed by Doppler ultrasound of lower limb vessels, MVC diagnosed by brain-computed tomography scan, and carotid doppler examination. Cases with a history of myocardial infarction, coronary artery bypass, peripheral arteries diseases, apoplexy incidents, electrocardiogram abnormalities that suggested angina pectoris or ischemia, or angiographically established CAD were recruited to the MVC group. All participants submitted full medical history and subjected to thorough clinical evaluation. Body weight (kg) and height (cm) of all subjects were taken and BMI was estimated. Blood pressure (mmHg) was estimated. After fasting for 12 hours samples were gathered from all patients into ethylenediaminetetraacetic acid tubes for plasma collection needed for routine biochemical tests and plain tubes for serum separation. Routine biochemical tests as glycated hemoglobin (HbA1C), fasting blood glucose (FBG), fasting insulin, lipid profiles as triglycerides, cholesterol, high-density lipoprotein-cholesterol (HDL-C), and serum creatinine were performed by the automatic biochemical analyzer (Olympus, AU 400, Tokyo, Japan). High-density lipoprotein-cholesterol, TC, and TG levels were utilized to calculate LDL-C. Insulin resistance (HOMA-IR) was calculated. Enzyme-linked immunosorbent assay (ELISA) kits from BioVendor GmbH in Heidelberg, Germany were utilized for serum levels evaluation of irisin (Cat# RAG005R), RBP4 (Cat# RAG018R), and leptin (Cat# RD191991199). ### Statistical analysis Data were presented as mean + standard deviation (SD) and manipulated with IBM SPSS Statistics for Windows, version 23 (IBM SPSS, IBM Corp., Armonk, NY, USA). Shapiro-Wilk test was utilized to evaluate normal data distribution. Comparisons between groups were made using one-way analysis of variance (ANOVA) then by least-significant difference test (LSD) for normally distributed variables and Mann-Whitney U-test for abnormally distributed variables. Correlations between different measured parameters made using Pearson’s correlations. Binary logistic regression analysis was utilized to find risk factors for macrovascular complications in T2DM. Data were statistically significant if *p*-values <0.05. ## Results Fasting blood glucose, fasting insulin, HOMA-IR, HbA1C, RBP4, and leptin values in T2DM cases without and with MVC complications were significantly elevated versus controls (*p*<0.0001). Cholesterol values were elevated in T2DM cases without MVC versus controls and T2DM with MVC (*p*=0.002 and *p*=0.022). Retinol-binding protein 4 values were significantly decreased in T2DM cases with MVC versus those without MVC (*p*<0.0001) HDL-C and irisin values in T2DM patients without and with MVC complications were significantly declined versus controls. Irisin levels were significantly declined in T2DM with MVC complications versus those without MVC (*p*=0.007) (Table 1). View this table: [Table 1](http://smj.org.sa/content/41/12/1369/T1) Table 1 Comparison of demographic and clinical characteristics and measured parameters in different studied groups. In all participants, there were negative associations between irisin levels and RBP4, FBG, insulin, HOMA-IR, HbA1C, cholesterol, triglyceride, LDL-C. Serum RBP4 showed positive correlations with FBG, insulin, HOMA-IR, and HbA1C, but negative correlations with HDL-C. Serum leptin had positive correlations with BMI, FBG, insulin, HOMA-IR, and HbA1C (Table 2). View this table: [Table 2](http://smj.org.sa/content/41/12/1369/T2) Table 2 Correlations between different inflammatory markers and measured parameters in all participants. Macrovascular complications patients were evaluated with binary logistic regression analysis in which groups were taken as dependent, and adipokines, lipid profile, glycemic control as independent variables. Irisin was protective against MVC in T2DM patients (β=0.014, 95% CI 0.000-0.387, *p*=0.012). While RBP4 (β=1.020, 95%CI 1.011-1.1020, *p*<0.0001) and cholesterol (β=0.965, 95%CI 0.981-1.000, *p*=0.049) were positive risk factors for MVC in T2DM patients (Table 3). View this table: [Table 3](http://smj.org.sa/content/41/12/1369/T3) Table 3 Binary regression analysis of risk factors in type 2 diabetes mellitus patients with macrovascular complications. ## Discussion Considering the pro-inflammatory state of diabetes, several adipokine inflammation-related factors as irisin, RBP4, and leptin in T2DM cases with and without MVC were analyzed. This study revealed a decrease in serum irisin in T2DM subjects with and without MVC that confirmed irisin’s potential role in glucose metabolism regulation. Serum irisin was significantly declined in T2DM with MVC versus without MVC. Type 2 DM cases with MVC have more inflammation in their vascular tissues than those without MVC. Zhang et al8 reported a significant decline in serum irisin in T2DM and showed a further decrease in serum irisin when MVC existed. Anastasilakis et al4 reported lower serum irisin in MI and CAD cases than controls. Changes in activity and expression of peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α) may explain irisin level reduction in T2DM cases. Irisin is stimulated by PGC-1α expression. Meanwhile, in T2DM, PGC-1α expression was down-regulated in skeletal muscles. Therefore, reduced serum irisin values in T2DM patients are caused by reduced PGC-1α synthesis and function in their muscles.9 Furthermore, the experimental animal model suggested that during ischemic cardiomyopathy, decreased FNDC5 expression in skeletal muscle is due to inflammatory cytokines.10 Low levels of irisin in T2DM with MVC cases in this research could be explained by that irisin energy hemostasis controlling effects as most secreted irisin used to control disturbed metabolism. In addition, in this study, significant negative associations between serum irisin and HbA1C, cholesterol, triglyceride, and LDL-C were found. Moreover, raised irisin values were related to a decreased risk of MVC in T2DM (β=0.014, 95%CI 0.000-0.387, *p*=0.012). Retinol-binding protein 4 is the chief circulatory transporter protein for retinol (vitamin A). Retinol-binding protein 4 was found to be genetically linked to T2DM risk. In this research, serum RBP4 values were significantly higher in T2DM cases with and without MVC than in controls. A case-control study showed that serum RBP4 values were more elevated in T2DM cases in comparison to the controls and RBP4 strongly related to LDL, therefore it is considered as a risk factor for CVS development.6 Kadoglou et al11 found that serum RBP4 values were higher in established carotid atherosclerosis cases than controls. Our data showed that serum RBP4 levels were higher in T2DM cases with MVC than T2DM cases without MVC. Zhang et al8 reported that RBP4 values were significantly increased in T2DM cases with MVC versus those without MVC. Declined expression of glucose transporter 4 in adipose tissue in a diabetic patient was related to increased RBP4 expression in adipose tissue and raised RBP4 serum values. Increased circulating RBP4 raised insulin resistance by suppressing insulin signaling in muscle and raising liver glucose output.8 The results of this study showed that RBP4 had negative associations with irisin and HDL-C but had positive associations with FBG and HbA1C. Moreover, increased RBP4 values were associated with increased MVC risk in T2DM (β=1.020, 95%CI 1.011-1.029, *p*<0.0001). Kwanbunjan1et al. found strong positive correlations between RBP4 and TG in T2DM cases moreover, they found a negative association between HDL-C and RBP4.12 Multiple fields of research have studied the correlation between leptin and diabetes mellitus and the results have been variant. High leptin values were reported related to adiposity and different CVD risk factors. Additionally, they are associated with IR and increased T2DM risk. In this study, the results revealed that serum leptin was higher in T2DM cases with and without MVC than in controls. Also, similar results were reported by Al Sheikh, who studied serum leptin levels in correlation with T2DM in a sample of Saudi males; she found a significant rise in serum leptin in the diabetic group versus non-diabetic group.7 In accordance with our results, the study of Memon et al13 studied serum values of adipocytokines and advanced glycation end products in diabetic and non-diabetic cases with myocardial infarction, and they reported that T2DM patients with or without MI had significantly higher leptin values than controls.13 In this study, leptin had positive associations with RBP4, BMI, FBG, and HbA1C. Similar results have been achieved by Memon et al,13 reported that there was a significant positive correlation between leptin and BMI and FBG in T2DM patients they explained their results by that inflammation is playing a pivotal role in cardiovascular complication the pathogenesis in T2DM. ### Study limitations This study has lack of registration of waist-hip circumference as an indicator of visceral obesity. Lastly, lack of estimation of highly sensitive CRP that is potential markers of cardiovascular diseases. In conclusion, possible beneficial anti-inflammatory properties of irisin and the harmful effects of RBP4 in T2DM patients with MVC. Association between irisin with markers of glucose homeostasis and lipid profile suggested that they may be a predictor of and protective factor against developing DM. 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