The NLRP3 inflammasome rs35829419 C>A polymorphism is associated with type 2 diabetes mellitus in Saudi Arabia ================================================================================================================= * Yahia A. Kaabi ## Abstract **Objectives:** To investigate the frequency of NLRP3 gene rs35829419 C>A single-nucleotide polymorphism (SNP) in a Saudi Arabian population from Jazan (Southwest Saudi Arabia) and test its potential association with type 2 diabetes mellitus (T2DM). **Methods:** This case-control study included 546 volunteers (271 patients with T2DM and 275 healthy controls) recruited from outpatient clinics at Jazan University Hospital and King Fahad Central Hospital in Jazan, Saudi Arabia, between December 2021 and July 2022. Genomic DNA was extracted from all samples and genotyped for the NLRP3 rs35829419 C>A SNP using TaqMan technology. The association between the NLRP3 rs35829419 polymorphism and T2DM was examined using logistic regression analysis. **Results:** Overall genotype distributions were 90.5% (CC), 9.3% (CA), and 0.2% (AA). The heterozygous CA genotype was more frequent in T2DM group (12.2%) compared to the control group (6.5%) and logistic regression analysis showed a statically significant association with T2DM risk under codominant (CA versus CC; odds ratio [OR]=1.99; 95% confidence interval [CI]= [1.11-3.61]; *p*=0.0270), and dominant (CA+AA versus CC; OR=2.05; CI=[1.16-3.75]; *p*=0.019) models of inheritance. **Conclusion:** This study revealed the frequency of NLRP3 rs35829419 C>A polymorphism in our population and showed a direct correlation between having the minor allele for A and having a higher risk of developing T2DM. This study highlights the significance of NLRP3 rs35829419 C>A polymorphism in the pathophysiology of T2DM. Keywords: * type 2 diabetes mellitus * inflammasome * NLRP3 rs35829419 C>A SNP * Saudi Arabia **O**ver the last 3 decades, type 2 diabetes mellitus (T2DM) has become a critical wellbeing threat affecting most populations worldwide. Saudi Arabia has the highest incidence rate of T2DM, comprising approximately 31.6% of the population.1 Peripheral insulin resistance and hyperglycemia are the main manifestations of T2DM. Despite the significant role of genetics in T2DM, the precise etiology of the disease remains poorly elucidated. However, in T2DM patients, elevated plasma levels of proinflammatory molecules suggest a central role of inflammation in the pathogenesis of insulin resistance and T2DM.2 In T2DM, production of reactive oxygen species (ROS), stimulated by high blood glucose and dyslipidemia, may lead to increased oxidative stress and chronic inflammation; the foundation for molecular processes resulting in T2DM and its complications.3 Inflammasomes are large multi-protein complexes that are assembled within stimulated immune cells upon sensing danger- or pathogen-associated molecular patterns, leading to the initiation of innate inflammatory response through activation of caspa-1 and pro-interleukin-1 beta (IL-1β).4 The nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) is the most frequently studied inflammasome among numerous inflammasome complexes.5 Activation of the NLRP3 protein by mitochondrial free radicals, ROS or cardiolipin leads to formation of the NLRP3 inflammasome complex, which triggers and regulates the innate immune response.6 Activation of the NLRP3 inflammasome by glucose and glycolipids in T2DM is closely associated with a chronic inflammatory state that may contribute to insulin resistance.7,8 The NLRP3 encoded by the NLRP3 gene, (contains 9 exons) found on the long (q) arm of chromosome-1.9 Mutations in the NLRP3 gene associated with several pathological conditions, where inflammation plays a role such as organ-specific autoimmune diseases and metabolic disorders.10 Until now, more than 60 single-nucleotide polymorphisms (SNPs) are found in the NLRP3 gene including rs35829419 C>A polymorphism (Q705K). The NLRP3 gene rs35829419 C>A SNP is a gain-of function polymorphism located within exon-3 of the NLRP3 gene, causing overactivity of the NLRP3 inflammasome, overproduction of bioactive interleukin (IL)-1β, and elevated basal proinflammatory phenotype.9 This SNP has not been directly linked to the T2DM risk factors. However, it has been linked to several autoimmune disorders including systemic lupus erythematosus, type1 diabetes mellitus, and Alzheimer’s disease.11 Therefore, the goal of this study was to explore the frequency of the NLRP3 gene rs35829419 C>A SNP in a Saudi Arabian population from Jazan, Saudi Arabia, and to test its potential association with T2DM or other anthropometric parameters. ## Methods The current case-control study involved 546 Saudi Arabian volunteers of both genders, ranging in age from 35-98 years. The samples were divided into 2 groups: 271 patients diagnosed with T2DM and 275 healthy non-diabetic individuals who served as controls. Volunteer samples were collected from the blood donation area and outpatient clinics at Jazan University Hospital and King Fahad Central Hospital in Jazan, Saudi Arabia, between December 2021 and July 2022. The inclusion criteria were: i) a Saudi Arabian male or female citizen; ii) ≥35 years old of age; III) with or without T2DM. Participant aged <35 years old, morbidly obese subjects, pregnant women, and patients with other form of diabetes were excluded from this study. Prior to sample collection, each participant signed a written informed consent form. This study adhered to the ethical standards outlined in the Declaration of Helsinki and was approved by the local Research Ethics Committee of Jazan University (REC-43/02/017). Before blood sample collection, basic demographic information, such as age, gender, weight, and height, were recorded. Body mass index (BMI) was calculated using body weight (kg) and heights (m) according the formula; BMI=kg/m2. Serum was tested for plasma glucose after an overnight fast using a colorimetric glucose oxidase-based technique. Plasma levels of glycated hemoglobin A1c (HbA1c) were measured using a turbidimetric immunoassay. The diagnosis of T2DM was established based on fasting plasma glucose (FPG) levels of ≥126 mg/dL or HbA1c readings of ≥6.5%. Each participant donated 5 mL of venous blood, which was drawn using the aseptic phlebotomy technique in an ethylenediaminetetraacetic acid-containing tube. Approximately 200 µL of whole blood was then used for genomic DNA isolation in a final elution buffer volume of 200 µL using the GeneJET gDNA purification kit (Thermo Fisher Scientific, Waltham, MA, USA). Immediately after DNA extraction, Thermofisher NanoDrop spectrophotometer was used to measure the amount of DNA found in each sample. The DNA concentration range was from 20-30 ng/µL. The A260/A280 ratio for each sample was also calculated to assess the purity of the extracted DNA; all samples had a ratio between 1.8-2.0. All DNA samples were then used directly for subsequent polymerase chain reaction (PCR) genotyping or stored frozen at -20°C. All DNA samples were genotyped for the NLRP3 rs35829419 C>A polymorphism in an ABI 7300 real-time polymerase chain reaction (RT-PCR) thermal cycler (Applied Biosystems) using TaqMan SNP Genotyping assay (ID: C_25648615_10; Thermo Fisher Applied Biosystems, Foster City, CA, USA). The reagents for this allelic discrimination assay included TaqMan primers and allele-specific probes labeled with fluorescein amidites and victoria fluorescent dyes for the detection of A and C alleles. The primers and probes were designed specifically for this assay to enable discrimination between the 2 alleles. Polymerase chain reaction was carried out in a 96-well plate in a total volume of 25 µL. Initially, 1.25 ul of the 20x TaqMan SNP genotyping assay reagent was mixed with 12.5 µL of the 2x TaqMan genotyping master mix and placed in each well. Next, 10 ng of the sample DNA diluted in 11.25 µL of water was added to the designated wells and mixed by gentle tapping. The 96-well plate was then briefly centrifuged to bring all the samples to the bottom of the tubes and transferred to a thermal cycler for RT-PCR. The PCR protocol for DNA amplification and polymorphism detection were comprised of 3 steps: I) pre-PCR read (30 seconds reading of baseline fluorescence at 60°C); II) DNA amplification (10 minutes of initial denaturation at 94°C, then 40 cycles of 15 seconds DNA denaturation at 92°C, and one minute extension step at 60°C); III) post-PCR read (30 seconds reading of final fluorescence at 60°C). For allelic discrimination, Sequence Detection System, version 3.0 software (Applied Biosystems, Foster City, CA, USA) was used to collect and analyze the data and assign allele calls for each sample. ### Statistical analysis GraphPad Prism 9 software (GraphPad Software, Inc., San Diego, CA, USA) was used. Continuous data were expressed as mean±standard deviation (SD) and were compared using Student’s t-test. Categorical data (such as gender and genotype) are presented as frequencies and percentages and compared using the Chi-square (χ2) test. Using a Chi-square test, Hardy-Weinberg equilibrium (HWE) analysis was carried out on the healthy control group to determine whether the NLRP3 rs35829419 C>A polymorphism frequencies were within equilibrium. Binary logistic regression analysis was used to assess the relationship between the NLRP3 rs35829419 C>A SNP and the risk of developing T2DM by calculating the odds ratios (ORs) at a 95% confidence interval (CI). All tests were 2-sided, and a *p*-value of <0.05 was considered significant. ## Results Table 1 summarizes the demographic baseline characteristics and blood glucose measurements of T2DM patients and healthy controls. No significant differences based on gender or age were found between the 2 groups (*p*>0.05). However, other demographic variables (weight, height, and BMI) showed significant differences (*p*<0.05). Furthermore, T2DM patients exhibited significantly greater levels of FPG (*p*<0.0001) and glycated hemoglobin (*p*<0.0001) than the control group. View this table: [Table 1](http://smj.org.sa/content/44/8/745/T1) Table 1 -Comparison of the demographic variables between type 2 diabetes mellitus patients and healthy control group. Genotypes distribution and allelic frequencies of for the NLRP3 rs35829419 C>A polymorphism in both diabetic and control groups are shown in Table 2. The frequency of the NLRP3 rs35829419 homozygous CC genotype was slightly higher in the healthy controls (93.5%) than in the T2DM group (87.4%). By contrast, the CA genotype was more frequent in the T2DM group (12.2%) than in the control group (6.5%). The AA genotype was extremely rare in our sample population; only one AA genotype was detected in the diabetic group, and no AA genotype was found among the controls. Allelic distribution also showed a higher frequency of the minor A allele in the T2DM group (6.5%) than in the control group (3.3%). The observed genotype frequencies in the control group were consistent with HWE (*p*=0.575). Logistic regression analysis revealed a statistically significant association between the NLRP3 rs35829419 C>A SNP and T2DM risk under codominant (CA versus CC; OR=1.99; 95% CI= [1.11-3.61]; *p*=0.0270), and dominant (CA+AA versus CC; OR=2.05; 95% CI= [1.16-3.75]; *p*=0.019) models of inheritance. This association was also demonstrated at the allelic level (A versus C; OR=2.04; 95% CI= [1.15-3.70]; *p*=0.016). View this table: [Table 2](http://smj.org.sa/content/44/8/745/T2) Table 2 -Allele change (frequency) of the nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 rs35829419 single-nucleotide polymorphism among type 2 diabetes mellitus and control group. To assess the effect of the NLRP3 rs35829419 C>A SNP on the basic parameters of the study groups, data were recalculated based on the NLRP3 rs35829419 polymorphism variants and assessed for significant differences between the means (Table 3). Based on our observations, we found that T2DM patients carrying the heterozygous CA genotype exhibited significantly higher weight and BMI compared to those with the CC genotype. No other statistically significant differences were observed. View this table: [Table 3](http://smj.org.sa/content/44/8/745/T3) Table 3 -Basic characteristics of studied groups by nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 rs35829419 genotype. ## Discussion In Saudi Arabia, T2DM is a common disorder and major health concern. Understanding the genetic background of T2DM will facilitate efficient disease control. Although the exact single genetic defect causing T2DM has not yet been reported, genome-wide association studies revealed hundreds of altered gene loci that may contribute to the development of T2DM.12 This study investigated the frequency of the NLRP3 rs35829419 C>A (Q705K) SNP in Saudi Arabia and its association with the risk of T2DM. Initially, results showed that the polymorphic A allele is a minor allele and was found in approximately 9.5% of the total study population. Different geographical regions showed different frequencies of this SNP, with the highest being reported in Europeans (11%) and the lowest in Japanese (2.2%), but not reported in some populations such as Chinese or African-Americans.13 This study found a higher frequency of the minor A allele in T2DM group than controls. Logistic regression analysis revealed a statistically significant association between NLRP3 rs35829419 C>A (Q705K) polymorphism and T2DM in our population. This previously unrevealed finding that emphasized the significance of the NLRP3 inflammasome and innate immunity in the development of T2DM. There is a growing body of research has demonstrated the fundamental connection between diabetes and the NLRP3 inflammasome. Initially, it has been found that the level of the NLRP3 inflammasome is an important indicator for metabolic threat that may lead to the development of T2DM.14 This was further confirmed by the phenotype of improved glucose tolerance and insulin sensitivity in NLRP3 gene knockout mice, that protected the mice from developing diabetes and obesity.15 Activation of the NLRP3 inflammasome causes a chronic low-grade systemic inflammation, which is a major contributor to insulin resistance, T2DM incidence, and its complications.14 In patients with diabetes, high blood glucose can activate the NLRP3 inflammasome via the ATP/P2X purinergic receptor 4 pathway.7 Additionally, glucose increases the expression of the thioredoxin interaction protein cofactor necessary for NLRP3 inflammasome activation.7 The NLRP3 rs35829419 C>A (Q705K) polymorphism is a gain-of-function mutation associated with increased inflammasome activity and increased basal systemic inflammatory state 15 which in turn may contribute to T2DM.16 Other SNPs found in NLRP3 gene, such as rs10754558 and rs4612666, have also been associated with T2DM risk.17 Apart from T2DM incidence, activation of the NLRP3 inflammasome has been linked to the development of the diabetes complications. The NLRP3 rs35829419 C>A polymorphism in particular has been linked to increased risk of T2DM complications such as macrovascular complications.18 Moreover, an inhibitory herbal medicine to the NLRP3 inflammasome has been found to improve diabetic peripheral neuropathy.19 In another study, activation of the NFLRP3 inflammasome has been linked to diabetic retinopathy.20 This study reported higher body weights and mass indices in diabetic patients carrying the minor A allele of the NLRP3 rs35829419 C>A polymorphism than in controls. This finding also suggests a possible role for this polymorphism in the regulation of fat metabolism and obesity, which by itself is associated with low-grade systemic inflammation and T2DM risk.21 Although there is no direct evidence of a connection between the NLRP3 gain-of-function polymorphism rs35829419 C>A and obesity, some studies have shown that activation of the NLRP3 inflammasome may contribute to adipocytes dysfunction and development of obesity.22 On the other hand, altered adipose tissue microenvironment in obesity can lead to NLFRP3 activation and initiation of systemic inflammation.23 ### Study limitations Owing to the scarcity of the studied genetic polymorphism in our population, the relatively small sample size is a major limiting factor that may have affected the statistical power of the study. Moreover, all patients were recruited from only one geographic area in Saudi Arabia (Jazan) with a genetically homogenous population, which may not reflect the entire Saudi Arabian population. Therefore, other studies with larger sample sizes recruited from different regions of Saudi Arabia are required to confirm the findings of this study. In conclusion, this study aimed to examine the frequency of the NLRP3 rs35829419 C>A polymorphism in a Saudi Arabian population and to test its possible association with T2DM. Here, we report that the minor A allele of the NLRP3 rs35829419 C>A SNP was associated with an increased risk of T2DM in our population. Additionally, T2DM patients carrying the minor A allele exhibited a significant increase in BMI compared to controls. This study highlights the importance of NLRP3 rs35829419 C>A polymorphism in the pathogenesis of T2DM. 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