PT  - JOURNAL ARTICLE
AU  - Meshkat, Zahra
AU  - Soleimanjahi, Hoorieh
AU  - Mahmoudi, Mahmoud
AU  - Mirshahabi, Hessam
AU  - Hassan, Zuhair M.
AU  - Ghaffari, Saeed R.
AU  - Sabokbar, Tayebeh
TI  - Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.
DP  - 2007 Oct 01
TA  - Saudi Medical Journal
PG  - 1511--1515
VI  - 28
IP  - 10
4099  - http://smj.org.sa/content/28/10/1511.short
4100  - http://smj.org.sa/content/28/10/1511.full
SO  - Saudi Med J2007 Oct 01; 28
AB  - OBJECTIVES: To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine.METHODS: The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and confirmed by colony-PCR, restriction enzyme analysis, and sequenced.RESULTS: The desired plasmid was sequenced and indicated 99% homology with those mentioned in the Genbank.CONCLUSIONS: The Iranian HPV16 E7 gene sequence is very similar to other sequences in the Genbank, and it can be used as a candidate gene in a therapeutic vaccine for Iranian patients with cervical cancer.