RT Journal Article
SR Electronic
T1 Effect of panaxatriol on hematogenesis and granulocyte-macrophage colony stimulating factor in radiation injured mice
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1791
OP 1795
VO 28
IS 12
A1 Liu, Fen Y.
A1 Wang, Jun N.
A1 Yu, Shu D.
A1 Wang, Bo
A1 Zhang, Ji D.
YR 2007
UL http://smj.org.sa/content/28/12/1791.abstract
AB OBJECTIVE: To assess the recovery effect of panaxatriol (PT) on myeloid hemopoiesis in radiation injured mice, and analyze the underlying mechanism.METHODS: This study was carried out in the Animal Center of Shandong University, Jinan, China, during March to September 2006. Forty-five inbred albino mice were separated randomly into 3 groups: control group, radiation group, and radiation + PT group (200 mg/kg/d, 3 weeks). Peripheral blood cells were detected by globuli meter, CD34+ cells in bone marrow were detected by flow cytometry, and the protein expression of granulocyte-macrophage colony stimulating factor (GM-CSF) was detected by immunocytochemistry.RESULTS: The numbers of peripheral blood cells and bone marrow CD34+ cells, and the expression of GM-CSF in the radiation group were lower than in the control group. After treatment with PT, the numbers of peripheral blood cells and CD34+ cells, and the expression of GM-CSF increased significantly.CONCLUSION: Panaxatriol can relieve myelosuppression induced by radiation injury. The abilities of regulating the expression of hemopoietic growth factor GM-CSF and promoting the maturation of bone marrow cells may be responsible for some of these beneficial effects.