RT Journal Article
SR Electronic
T1 Proteomic analysis of clear cell renal cell carcinoma. Identification of potential tumor markers
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 525
OP 532
VO 31
IS 5
A1 Chuan Y. Sun
A1 Ya C. Zang
A1 Yu X. San
A1 Wei Sun
A1 Liang Zhang
YR 2010
UL http://smj.org.sa/content/31/5/525.abstract
AB OBJECTIVE: To identify new tumor specific proteins of renal cell carcinoma (RCC) using proteomic analysis.METHODS: Nine renal cell carcinomas were resected and these surgical operations were carried out from June 2007 to September 2008 in the Urology Department of the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. The cancer tissues and para-cancer normal tissues were preserved in liquid nitrogen. We analyzed the RCC tissues and para-cancer normal tissues by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The 16 differentially expressed protein spots (p<0.05 by Student t-test) were identified by matrix assisted laser desorption ionization/time of flight mass spectrometry and tandem mass spectrometry.RESULTS: Six proteins were down regulated and 10 proteins were up regulated in clear cell RCC compared with corresponding normal kidney tissue. The down regulated proteins were calbindin, ester hydrolase C11orf54, alcohol dehydrogenase, ammecr1-like protein, adenosine diphosphate (ADP)/adenosine-5'-triphosphate (ATP) translocase 3 and leucine-tyrosine-arginine (LYR) motif-containing protein 5. The up regulated proteins were hypoxia inducible domain family member 1A, glutathione S-transferase P, thioredoxin-dependent peroxide reductase, peroxiredoxin-6, CD2-associated protein, annexin A5, gamma-enolase, retinal dehydrogenase 1, vimentin, and protein-glutamine gamma-glutamyltransferase 2.CONCLUSION: The data provide potential tumor markers for diagnosis of RCC.