PT  - JOURNAL ARTICLE
AU  - Bavi, Prashant P.
AU  - Abubaker, Jehad A.
AU  - Jehan, Zeenath D.
AU  - Al-Jomah, Naif A.
AU  - Siraj, Abdul K.
AU  - Al-Harbi, Sayer R.
AU  - Atizado, Valerie L.
AU  - Abduljabbar, Alaa S.
AU  - Alhomoud, Samar J.
AU  - Ashari, Luai H.
AU  - Al-Dayel, Fouad H.
AU  - Uddin, Shahab
AU  - Al-Kuraya, Khawla S.
AU  - Alsanea, Nasser A.
TI  - Colorectal carcinomas from Middle East. Molecular and tissue microarray analysis of genomic instability pathways
DP  - 2008 Jan 01
TA  - Saudi Medical Journal
PG  - 75--80
VI  - 29
IP  - 1
4099  - http://smj.org.sa/content/29/1/75.short
4100  - http://smj.org.sa/content/29/1/75.full
SO  - Saudi Med J2008 Jan 01; 29
AB  - OBJECTIVE: To evaluate the overall incidence of microsatellite instability (MSI), hereditary non polyposis colorectal cancer, and tumor supressor gene (TP53) mutations in Saudi colorectal carcinomas.METHODS: We studied the MSI pathway in Saudi colorectal cancers (CRC) from 179 unselected patients using 2MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8.RESULTS: Of the 150 colorectal carcinomas analyzed for MSI, 16% of the tumors showed high level instability (MSI-H), 19.3% had low-level instability (MSI-L) and the remaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group (p=0.0217). In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consanguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied.CONCLUSION: A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further.