RT Journal Article
SR Electronic
T1 Colorectal carcinomas from Middle East. Molecular and tissue microarray analysis of genomic instability pathways
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 75
OP 80
VO 29
IS 1
A1 Bavi, Prashant P.
A1 Abubaker, Jehad A.
A1 Jehan, Zeenath D.
A1 Al-Jomah, Naif A.
A1 Siraj, Abdul K.
A1 Al-Harbi, Sayer R.
A1 Atizado, Valerie L.
A1 Abduljabbar, Alaa S.
A1 Alhomoud, Samar J.
A1 Ashari, Luai H.
A1 Al-Dayel, Fouad H.
A1 Uddin, Shahab
A1 Al-Kuraya, Khawla S.
A1 Alsanea, Nasser A.
YR 2008
UL http://smj.org.sa/content/29/1/75.abstract
AB OBJECTIVE: To evaluate the overall incidence of microsatellite instability (MSI), hereditary non polyposis colorectal cancer, and tumor supressor gene (TP53) mutations in Saudi colorectal carcinomas.METHODS: We studied the MSI pathway in Saudi colorectal cancers (CRC) from 179 unselected patients using 2MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8.RESULTS: Of the 150 colorectal carcinomas analyzed for MSI, 16% of the tumors showed high level instability (MSI-H), 19.3% had low-level instability (MSI-L) and the remaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group (p=0.0217). In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consanguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied.CONCLUSION: A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further.