RT Journal Article
SR Electronic
T1 Combined effect of a mixture of tetracycline, acid, and detergent, and Nisin against <em>Enterococcus faecalis</em> and <em>Actinomyces viscosus</em> biofilms
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 211
OP 215
DO 10.15537/smj.2015.2.9947
VO 36
IS 2
A1 Balto, Hanan A.
A1 Shakoor, Zahid A.
A1 Kanfar, Maha A.
YR 2015
UL http://smj.org.sa/content/36/2/211.abstract
AB Objectives: To evaluate the combined effect of a mixture of tetracycline, acid, and detergent (MTAD) and Nisin against Enterococcus faecalis (E. faecalis) and Actinomyces viscosus (A. viscosus) biofilms.Methods: This study was conducted between June and December 2013 in collaboration with Dental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. Single-species biofilms (n=9/species/observation period) were generated on membrane filter discs and subjected to 5, 10, or 15 minute incubation with MTADN (MTAD with 3% Nisin), 5.25% sodium hypochlorite (NaOCl), or normal saline. The colony forming units were counted using the Dark field colony counter.Results: A 100% bactericidal effect of 5.25% NaOCl was noted during the 3 observation periods; a significant reduction (p=0.000) in mean survival rates of E. faecalis (77.3+13.6) and A. viscosus (39.6+12.6) was noted after 5 minutes exposure to MTADN compared with normal saline (78000000+5291503) declining to almost no growth after 10 and 15 minutes. The survival rates of the E. faecalis and A. viscosus biofilm were no different after treatment with MTADN and 5.25% NaOCl at the 3 observation periods (p=1.000).Conclusion: A combination of MTAD and Nisin was as effective as NaOCl against E. faecalis and A. viscosus biofilms.