RT Journal Article
SR Electronic
T1 Urinary trypsin inhibitor treatment ameliorates acute lung and liver injury resulting from sepsis in a rat model
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 368
OP 373
VO 29
IS 3
A1 Zhou, Li-Wen
A1 Wang, Yan-Lin
A1 Yan, Xue-Tao
A1 He, Xiang-Hu
YR 2008
UL http://smj.org.sa/content/29/3/368.abstract
AB OBJECTIVE: To evaluate the protective effect of urinary trypsin inhibitor UTI on acute lung and liver injury in rat model induced by sepsis with infra-abdominal infection.METHODS: This study was performed in the University of Wuhan, Wuhan, China in May 2007. Sepsis models were made by cecal ligation and puncture CLP in Sprague-Dawley rats. Forty rats were randomly divided into sham, CLP, CLP/UTI I 20u/g and CLP/UTI II 50u/g groups, with 10 rats in each. All of them were sacrificed 12 hours after CLP. The mean arterial pressure MAP, heart rate HR, the wet-to-dry lung weight ratio W/D was measured and venous blood was collected for assaying tumor necrosis factor-α TNF-α, interleukin-10 IL-10, alanine aminotransferase ALT, aspartate aminotransferase AST and lactic acid. Superoxide dismutase SOD, malondialdehyde MDA and expression of inducible nitric oxide synthase iNOS mRNA in lung and hepatic tissues were examined.RESULTS: Compared with the CLP group, MAP and HR in 50u/g UTI treated rats was stable p<0.01. Marked elevation levels of W/D ratio were lowered after administration of 50u/g UTI p<0.01. Treatment with 50u/g UTI prevented marked elevation in MDA, ALT, AST, TNF-α, lactic acid levels, expression of iNOS mRNA, and elevated IL-10 and SOD activity p<0.01.CONCLUSION: Urinary trypsin inhibitor has a protective effect against sepsis. Its action mechanisms are probably involved in the inhibition of inflammatory factor production and suppression of lipid peroxidation and iNOS mRNA expression.