RT Journal Article
SR Electronic
T1 Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1303
OP 1308
VO 31
IS 12
A1 Zhang, Peng
A1 Xu, You-jia
A1 Zhao, Dong-Yang
A1 Ma, Yong
A1 Xiao, Li
A1 Feng, Yu-Shuang
A1 Du, Ben-Cai
A1 Qian, Zhong-Ming
A1 Li, Kai
YR 2010
UL http://smj.org.sa/content/31/12/1303.abstract
AB OBJECTIVE: To address whether hepcidin functions in bone metabolism.METHODS: This study was carried out in the Laboratory of Radiation Medicine and Public Health of Soochow University, and the Laboratory of the Second Affiliated Hospital of Soochow University, Suzhou, China, from September 2009 to July 2010. The positive expression of ferroportin-1 (Fpn-1) was detected by reverse transcriptase-polymerase chain reaction. After the treatment with distilled water (control group) and hepcidin (25noml/L, 50noml/L, 100noml/L), the fluorescence intensity related to intracellular iron concentration of a human fetal osteoblast cell line (hFOB 1.19) was measured by a confocal laser scanning microscope. A 3-(4,5- dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide assay, and Von Kossa staining was performed to evaluate cell proliferation and mineralization in cultured hFOB 1.19 cells.RESULTS: This study revealed a high level expression of Fpn-1 in hFOB 1.19. On the basis of which, it was found that 25noml/L, 50noml/L, 100noml/L hepcidin could promote the fluorescence intensity related to intracellular iron concentration and mineralization in hFOB 1.19 in a dose-dependent manner (p<0.05), but hepcidin had no effect on FOB 1.19 proliferation (p>0.05).CONCLUSION: The hepcidin-ferroportin signal pathway may function in the osteoblast cell line of hFOB 1.19 cells. It is also suggested that cross-talk between iron and calcium homeostasis may play a role in bone metabolism in responding to hepcidin activation.