PT  - JOURNAL ARTICLE
AU  - Dai, Yong
AU  - Sui, Weiguo
AU  - Lan, Huijuan
AU  - Yan, Qiang
AU  - Huang, He
AU  - Huang, Yuanshuai
TI  - Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy
DP  - 2008 Oct 01
TA  - Saudi Medical Journal
PG  - 1388--1393
VI  - 29
IP  - 10
4099  - http://smj.org.sa/content/29/10/1388.short
4100  - http://smj.org.sa/content/29/10/1388.full
SO  - Saudi Med J2008 Oct 01; 29
AB  - OBJECTIVE: To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA).METHODS: We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences.RESULTS: This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests.CONCLUSION: Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.