RT Journal Article
SR Electronic
T1 Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1388
OP 1393
VO 29
IS 10
A1 Dai, Yong
A1 Sui, Weiguo
A1 Lan, Huijuan
A1 Yan, Qiang
A1 Huang, He
A1 Huang, Yuanshuai
YR 2008
UL http://smj.org.sa/content/29/10/1388.abstract
AB OBJECTIVE: To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA).METHODS: We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences.RESULTS: This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests.CONCLUSION: Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.