PT  - JOURNAL ARTICLE
AU  - Kesici, Ugur
AU  - Kesici, Sevgi
AU  - Polat, Erdal
AU  - Agca, Birol
AU  - Turkmen, Ulku A.
AU  - Ozcan, Deniz
AU  - Sari, Musa K.
TI  - Effects of intra-abdominal pressure increase on intestinal ischemia and bacterial translocation in experimental sepsis model.
DP  - 2011 Aug 01
TA  - Saudi Medical Journal
PG  - 813--817
VI  - 32
IP  - 8
4099  - http://smj.org.sa/content/32/8/813.short
4100  - http://smj.org.sa/content/32/8/813.full
SO  - Saudi Med J2011 Aug 01; 32
AB  - OBJECTIVES: To investigate the safety of laparoscopic intervention for diagnosis and treatment at 8 mm Hg pressure in one-hour period on acute peritonitis related intra-abdominal sepsis model.METHODS: In this study, we included 32 female Wistar-Albino rats, weighing 250 ± 20 g, and divided them into 4 groups. This study was conducted in Istanbul University Experimental Medical Research Institution (DETAE) laboratory from April to May 2009. Intra-abdominal sepsis was created with intraperitoneal (i.p.) one mL (109 CFU/mL) Escherichia coli (E. coli) injection, and pneumoperitoneum was formed with CO2 insufflation at 8 mm Hg pressure for one hour i.p. The rats were administered with: Group 1 - one mL i.p. isotonic saline; Group 2 - one mL i.p. isotonic saline + pneumoperitoneum; Group 3 - i.p E. coli; and Group 4 - i.p. E.coli + pneumoperitoneum. Data were analyzed using the Statistical Package for Social Sciences version 15 for Windows (SPSS Inc, Chicago, IL, USA).RESULTS: Fever and leukocyte values were considered high in Groups 3 and 4 compared with Groups 1 and 2 (p=0.001). The administered reproduction ratio of the E. coli strain was determined as 0% in Groups 1 and 2, and 100% in Groups 3 and 4.CONCLUSIONS: In this study, as pneumoperitoneum was formed for one hour at 8 mm Hg pressure, in case of intra-abdominal derived sepsis where emergency intervention is needed, we consider that laparoscopic approaches with low pressure may be used safely for diagnosis and treatment.