RT Journal Article
SR Electronic
T1 Leptin expression by heterotopic ossification-isolated tissue in rats with Achilles' tenotomy
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 605
OP 610
VO 30
IS 5
A1 Jun-Chang Xu
A1 Tao Wu
A1 Gui-Hua Wu
A1 Zhao-Ming Zhong
A1 Yong-Zhi Tang
A1 Jian-Ting Chen
YR 2009
UL http://smj.org.sa/content/30/5/605.abstract
AB OBJECTIVE: To set up heterotopic ossification (HO) models according to McClure and determine whether leptin messenger ribonucleic acid (mRNA) and protein are expressed in HO-isolated tissue.METHODS: This study was performed in the Department of Spine and Orthopedics, Southern Medical University, Guangzhou, China from November 2007 to June 2008. Thirty-six male rats were randomly divided into sham, partial achilles' tenotomy (PAT), and achilles' tenotomy (AT) groups, with 12 rats in each group. X-ray and histological examination were carried out to ensure the formation of HO at 5 and 10 weeks after operation. Specimens from achilles tendons and surrounding tissue were taken and processed. Meanwhile, the expression of leptin mRNA (5 and 10 weeks) and protein (10 weeks) in achilles tendons and the surrounding tissue were examined respectively using reverse transcription-polymerase chain reaction assay and immunohistochemical methods.RESULTS: There were no leptin mRNA and protein expression in the sham and a weak expression in the PAT of Achilles tendons and surrounding tissue, whereas there was strong expression in the AT group.CONCLUSION: Leptin is involved in the formation of HO, its mechanisms is related to induction of bone formation and maturation through a series of cellular events, including: proliferation/differentiation of many kinds of cells, collagen synthesis, mineralization, and vascularization of the extracellular matrix.