RT Journal Article
SR Electronic
T1 The effect of repeated diazepam administration on myocardial function in the ischemia-reperfused isolated rat heart
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 755
OP 759
VO 30
IS 6
A1 Dareuosh Shackebaeit
A1 Bijan Kayhanie
A1 Aliashraft Godinie
A1 Aliasghar Pourshanazari
A1 Sohylae Reshadat
YR 2009
UL http://smj.org.sa/content/30/6/755.abstract
AB OBJECTIVE: To evaluate whether repeated diazepam administration affects the heart in ischemia- reperfusion.METHODS: This study was performed at the Medical Biology Research Center, Kermanshah, Iran, from March to September 2008. Four groups of rats were subjected to a daily injection of diazepam (group 1 [0.5 mg/kg for 21 days], group II [2.5 mg/kg for 5 days], and group III [5 mg/kg for 5 days] intraperitoneally), and saline solution (21 days) in the control groups. Isolated, perfused hearts were subjected to 40 minutes global ischemia, and 45 minutes reperfusion. The left ventricular developed pressure (LVDP), heart rate, and coronary flow were measured. Rate pressure product (RPP) was calculated. In reperfusion, released lactate dehydrogenase (LDH) enzyme in effluent was measured.RESULTS: It was observed that the recovery of the RPP and LVDP in reperfusion significantly decreased in the test group III (n=9) in comparison to the control (n=8). During the reperfusion period, the released LDH significantly increased in test group II (n=8) and group III in comparison with the control.CONCLUSION: The results show that repeated administration of diazepam (5 mg/kg for 5 days) reduced the cardiac performance in reperfusion, and significantly exacerbated the ischemia-reperfusion injury. It is probably mediated by the changing of cardiac susceptibility in ischemia due to repeated administration of diazepam. The results show that repeated administration of diazepam (5 mg/kg for 5 days) reduced the cardiac performance in reperfusion, and significantly exacerbated the ischemia-reperfusion injury. It is probably mediated by the changing of cardiac susceptibility in ischemia due to repeated administration of diazepam.