RT Journal Article
SR Electronic
T1 Comparison of xanthine oxidase levels in synovial fluid from patients with rheumatoid arthritis and other joint inflammations
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1422
OP 1425
VO 30
IS 11
A1 Nadjet  Hanachi
A1 Noureddine Charef
A1 Abderrahmane Baghiani
A1 Seddik  Khennouf
A1 Yacine  Derradji
A1 Sabah Boumerfeg
A1 Daoud Harzallah
A1 Lekhmici Arrar
YR 2009
UL http://smj.org.sa/content/30/11/1422.abstract
AB OBJECTIVE: To search whether xanthine oxido-reductase (XOR) present in the synovium is also liberated, to determine its activity in synovial fluid and to establish a possible relationship between XOR levels in rheumatoid arthritis (RA) and non-RA patients.METHODS: This study was carried out in the Laboratory of Immunology, University Ferhat Abbas, Setif, Algeria from 2001-2008. This study is a retrospective controlled study matching cases with RA to non rheumatoid joint inflammations. Synovial fluid (SF) samples were collected with consent of the patients, at Setif University Hospital, from adults suffering from RA (n=36) or only with joint inflammations (n=52). After its detection in SF with indirect enzyme-linked immunosorbent assay (ELISA) and dot-immunobinding, using anti-bovine XOR as first antibodies, XOR was assayed with capture ELISA.RESULTS: Xanthine oxidoreductase is found in all studied SF. Capture ELISA showed levels up to 0.762 and 0.143 mg/mL in SF of RA and other joint inflammations patients, respectively. In most cases, more than 50% of synovial XOR is present as oxidase form. Positive correlation was observed between enzyme level and the disease severity since RA patients had a significantly high enzyme amount compared to patients with other less severe arthritic pathologies.CONCLUSION: These results suggest that the enzyme could well be involved in joint inflammation probably by producing reactive oxygen species.