RT Journal Article SR Electronic T1 The effect of regular training on plasma cytokines response in healthy and diabetic rats JF Saudi Medical Journal JO Saudi Med J FD Prince Sultan Military Medical City SP 1390 OP 1394 VO 30 IS 11 A1 Bonyadi, Mohammadreza A1 Badalzadeh, Reza A1 Mohammadi, Mustafa A1 Poozesh, Shahindokht A1 Salehi, Iraj YR 2009 UL http://smj.org.sa/content/30/11/1390.abstract AB OBJECTIVE: To evaluate the effect of regular swimming exercise on plasma levels of tumor necrosis factor (TNF)-a, interleukin (IL)-1B and of IL-6 cytokines in healthy and diabetic rats.METHODS: In this study, carried out from March 2008 to March 2009 in the Drug Applied Research Center, Tabriz, Iran, 40 Wistar rats (250-300g) were randomly divided into 4 groups (n=10): control-sedentary, control-exercised, diabetic-sedentary, and diabetic-exercised. Diabetes was induced by a single injection of streptozotocin (50mg/kg, intraperitoneally). The exercise protocol consisted of swimming (one hour/day, and 5 days/week) for 8 weeks. The plasma cytokines were measured by using specific kits and the enzyme-linked immunosorbent assay method.RESULTS: The findings of this study showed that the mean difference of IL-6 was significant among the groups, and that regular swimming increased the plasma levels of IL-6 to 9-times in healthy rats and to 23-times in diabetic ones (p=0.000, F (3,31)=54.79). However, there were no significant differences in IL-1B, and TNF-a levels among the groups.CONCLUSION: According to findings of this study, regular exercise causes an increase in plasma levels of IL-6, and this enhancement is much higher in diabetics rather than healthy rats. Thus, by increasing direct absorption of blood glucose by skeletal muscle, IL-6 can have a beneficial role in continuing the activities of diabetic patients.