RT Journal Article
SR Electronic
T1 The effect of regular training on plasma cytokines response in healthy and diabetic rats
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1390
OP 1394
VO 30
IS 11
A1 Bonyadi, Mohammadreza
A1 Badalzadeh, Reza
A1 Mohammadi, Mustafa
A1 Poozesh, Shahindokht
A1 Salehi, Iraj
YR 2009
UL http://smj.org.sa/content/30/11/1390.abstract
AB OBJECTIVE: To evaluate the effect of regular swimming exercise on plasma levels of tumor necrosis factor (TNF)-a, interleukin (IL)-1B and of IL-6 cytokines in healthy and diabetic rats.METHODS: In this study, carried out from March 2008 to March 2009 in the Drug Applied Research Center, Tabriz, Iran, 40 Wistar rats (250-300g) were randomly divided into 4 groups (n=10): control-sedentary, control-exercised, diabetic-sedentary, and diabetic-exercised. Diabetes was induced by a single injection of streptozotocin (50mg/kg, intraperitoneally). The exercise protocol consisted of swimming (one hour/day, and 5 days/week) for 8 weeks. The plasma cytokines were measured by using specific kits and the enzyme-linked immunosorbent assay method.RESULTS: The findings of this study showed that the mean difference of IL-6 was significant among the groups, and that regular swimming increased the plasma levels of IL-6 to 9-times in healthy rats and to 23-times in diabetic ones (p=0.000, F (3,31)=54.79). However, there were no significant differences in IL-1B, and TNF-a levels among the groups.CONCLUSION: According to findings of this study, regular exercise causes an increase in plasma levels of IL-6, and this enhancement is much higher in diabetics rather than healthy rats. Thus, by increasing direct absorption of blood glucose by skeletal muscle, IL-6 can have a beneficial role in continuing the activities of diabetic patients.