TY - JOUR T1 - Variation of M3 muscarinic receptor expression in different prostate tissues and its significance JF - Saudi Medical Journal JO - Saudi Med J SP - 1010 LP - 1016 VL - 30 IS - 8 AU - Wei Song AU - Mingzhen Yuan AU - Shengtian Zhao Y1 - 2009/08/01 UR - http://smj.org.sa/content/30/8/1010.abstract N2 - OBJECTIVE: To detect the expression of the muscarinic receptor (M receptor) in different prostate tissues and analyze the role of its subtype in prostatic oncogenesis.METHODS: Thirty-six cases of normal prostate and benign prostatic hyperplasia, and 8 cases of prostatic tumor, were used in this study from the Shandong University, Shandong, China, between 2003-2006. The protein expressions of M1, M2, and M3 receptors in each group were determined by Western-blotting. The gene expressions of the M3 receptor and vascular endothelial growth factors (VEGF) in each group were determined by reverse transcriptase-polymerase chain reaction.RESULTS: The protein and gene expressions of the M3 receptor in the prostatic carcinoma group were higher than that of benign prostatic hyperplasia group (p=0.0001) and normal prostate group (p=0.0001). The M3 receptor and VEGF showed positive straight-line correlations of gene expressions with the 3 groups (r=0.4999, p=0.0001).CONCLUSION: The M3 receptor may have a close relationship with prostatic oncogenesis. ER -