PT  - JOURNAL ARTICLE
AU  - Althubyani, Sarah A.
AU  - Alkhuriji, Afrah F.
AU  - Al Omar, Suliman Y.
AU  - El-khadragy, Manal F.
TI  - A preliminary study of cytokine gene polymorphism effects on Saudi patients with colorectal cancer
AID  - 10.15537/smj.2020.12.25543
DP  - 2020 Dec 01
TA  - Saudi Medical Journal
PG  - 1292--1300
VI  - 41
IP  - 12
4099  - http://smj.org.sa/content/41/12/1292.short
4100  - http://smj.org.sa/content/41/12/1292.full
SO  - Saudi Med J2020 Dec 01; 41
AB  - Objectives: To determine the possible associations of polymorphisms in interleukin (IL)-8 (rs4073 T/A), IL-10 (rs1800896 A/G), IL-22 (rs1179251 C/G and rs2227485 C/T), IL-27 (rs17855750 T/G), and transforming growth factor beta 1 (TGFß1) (rs1800469 C/T) with colorectal cancer (CRC) susceptibility in Saudi patients.Methods: The case-control study was carried out between July 2019 and January 2020 in King Khaled University Hospital, Riyadh, Saudi Arabia. A total of 70 patients with CRC and 70 healthy controls were included in the study. Single nucleotide polymorphisms of promoter regions were determined using TaqMan genotyping assays.Results: A statistically significant reduction in CRC risk was identified for carriers of the IL-10 (rs1800896 A/G) AG genotype, IL-22 (rs1179251 C/G) G allele, IL-27 (rs17855750 T/G) G allele and TGFß1 (rs1800469 C/T) CT and TT genotype. While IL-10 (rs1800896 A/G) AA genotype and TGFß1 (rs1800469 C/T) CC genotype were significantly associated with increased susceptibility to CRC. No significant associations were identified between the cytokine polymorphisms of IL-8 (rs4073 T/A) and IL-22 (rs2227485 C/T), and CRC risk.Conclusion: Our findings indicate a significant impact of IL-10 (rs1800896 A/G), IL-22 (rs1179251 C/G), IL-27 (rs17855750 T/G) and TGF-ß1 (rs1800469 C/T) polymorphisms on risk of CRC; while the IL-8 (rs4073 T/A) and IL-22 (rs2227485 C/T) and polymorphisms were not associated with CRC risk.