PT  - JOURNAL ARTICLE
AU  - Nasr, Ashraf Y.
TI  - Morphological, biochemical, histological, and ultrastructural protective effects of misoprostol on cisplatin induced-hepatotoxicity in adult male rats
DP  - 2013 Dec 01
TA  - Saudi Medical Journal
PG  - 1237--1247
VI  - 34
IP  - 12
4099  - http://smj.org.sa/content/34/12/1237.short
4100  - http://smj.org.sa/content/34/12/1237.full
SO  - Saudi Med J2013 Dec 01; 34
AB  - OBJECTIVE: To investigate the possible protective effect of misoprostol on cisplatin-induced hepatotoxicity.METHODS: Four-equal sized groups (control, cisplatintreated, misoprostol-treated, combined misoprostol, and cisplatin-treated) adult male Wistar rats (6 each) were used in this study. Body weight, liver weight, and liver weight/body weight ratio was calculated. Blood samples were obtained from the hearts of rats to determine the levels of total serum bilirubin (TSB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and albumin. Liver specimens were prepared for both light and electron microscopes. The study was carried out between June 2012 and April 2013 at the Anatomy Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt, and the Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.RESULTS: A single cisplatin dose (7.5 mg/kg intraperitoneally) resulted in significant elevation of AST, ALT, and TSB serum levels, and a significant reduction of serum albumin level, body weight, liver weight, and liver weight/body weight ratio. A combination of misoprostol (200 μg/kg/day) with cisplatin improved most of the previous parameters. Examination of specimens by both light and electron microscopes revealed pericentral hepatic necrosis, periportal fibrosis, dilatation, and congestion of central vein and blood sinusoids, diminished glycogen content, degenerated mitochondria, vesicular dilated rough endoplasmic reticulum, and nuclear changes in cisplatin-treated rats. Oral intake of misoprostol with cisplatin improved many of these changes.CONCLUSION: The results indicate that misoprostol may have a protective effect on cisplatin-induced hepatotoxicity.