PT  - JOURNAL ARTICLE
AU  - Judong Luo
AU  - Xialin Chen
AU  - Guanglie Chen
AU  - Xifa Zhou
AU  - Xujing Lu
AU  - Yang Ling
AU  - Shuyu Zhang
AU  - Wei Zhu
AU  - Jianping Cao
TI  - Dihydroartemisinin induces radiosensitivity in cervical cancer cells by modulating cell cycle progression
DP  - 2013 Mar 01
TA  - Saudi Medical Journal
PG  - 254--260
VI  - 34
IP  - 3
4099  - http://smj.org.sa/content/34/3/254.short
4100  - http://smj.org.sa/content/34/3/254.full
SO  - Saudi Med J2013 Mar 01; 34
AB  - OBJECTIVE: To investigate the radiosensitizing effects of dihydroartemisinin (DHA) and its underlying mechanisms in cervical cancer cells.METHODS: This experimental study was conducted between May 2009 and August 2012 in the School of Radiation Medicine and Protection, Soochow University, Suzhou, China. HeLa and Siha cells were assigned as the control group and DHA as treated group. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, clonogenic assay, cell cycle analysis, and apoptosis analysis were carried out in 2 cell lines of both groups.RESULTS: The inhibitory effect of DHA on the HeLa and Siha cell lines was dependent on both concentration and time. Dihydroartemisinin increased the radiosensitivity of HeLa cells, but not of Siha cells. Apoptosis and the gap2/mitosis (G2/M) phase transition induced by x-irradiation was enhanced by DHA treatment in HeLa cells. Irradiation, combined with DHA, decreased Wee1 expression while increasing Cyclin B1 expression in HeLa cells.CONCLUSION: Dihydroartemisinin potently abrogates G2 checkpoint control in HeLa cells. It can relieve the G2/M arrest induced by irradiation; thus, it can be used as an effective radiosensitizer, which will probably promote the entry of more irradiation-damaged cells into mitosis.