PT - JOURNAL ARTICLE AU - Hamid, Mohamed E. AU - Al Azraqi, Tariq A. AU - Joseph, Martin R. AU - Al-Hakami, Ahmed M. TI - Isolation of a rare Nocardia wallacei from an HIV-positive patient with pulmonary infection in southern Saudi Arabia DP - 2013 Jun 01 TA - Saudi Medical Journal PG - 644--647 VI - 34 IP - 6 4099 - http://smj.org.sa/content/34/6/644.short 4100 - http://smj.org.sa/content/34/6/644.full SO - Saudi Med J2013 Jun 01; 34 AB - This study aimed to clarify the taxonomic position of an actinomycete isolated from an HIV-positive male patient with pulmonary complications in Asir, southern region of Saudi Arabia. The strain was found to have phenotypic properties typical of nocardiae and 16S rRNA gene analysis clustered the isolate with Nocardia wallacei (accession KC677696) in the phylogenetic branch of the amikacin resistance Nocardia transvalensis complex. We consider that nocardiosis is usually missed or misdiagnosed clinically and recognition of these bacteria based on phenotypic tests is strenuous, but definitive identification is attainable by molecular methods.