RT Journal Article
SR Electronic
T1 The relationship between cytokine gene polymorphism and unexplained recurrent spontaneous abortion in Saudi females
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 484
OP 489
VO 34
IS 5
A1 Afrah F. Alkhuriji
A1 Ahmad R. Alhimaidi
A1 Zeneb A. Babay
A1 Arjumand S. Wary
YR 2013
UL http://smj.org.sa/content/34/5/484.abstract
AB OBJECTIVE: To investigate the relationships between unexplained recurrent spontaneous abortion (RSA) and single nucleotide polymorphisms tumor necrosis factor-alpha (TNF-alpha) (-238 G/A, -308 G/A), interleukin (IL)-6 (-634 G/C) and IL-10 (-592 C/A) in the promoter region of 3 different interleukin (TNF-alpha, IL-6, and IL-10) genes.METHODS: The study group comprised 65 women (mean age: 34.1+/-6.2; range: 15-45 years) with unexplained RSA, consecutively referred to the Recurrent Abortion Clinic, King Khaled University Hospital, Riyadh, Kingdom of Saudi Arabia from January 2010 to January 2011. The control group consisted of 65 females with at least 2 successful pregnancies and no history of abortion. Blood samples were drawn and deoxyribonucleic acid (DNA) was extracted using Puregene DNA purification kit. Utilizing polymerase chain reaction, the promoter region was amplified and sequenced on an Applied Biosystems Integrated sequencer to study the polymorphic sites of interest. All polymorphisms were identified in the case and control samples.RESULTS: A significant association was identified only between the -308 G/A polymorphism in the TNF-alpha gene promoter and the occurrence of unexplained RSA, and there was no significant association with other positions.CONCLUSION: The TNF-alhpa gene polymorphism at position -308 could be a genetic predisposing factor for unexplained RSA.