PT  - JOURNAL ARTICLE
AU  - Li, Jin-Ming
AU  - Zhao, Rong-Hua
AU  - Li, Shao-Tang
AU  - Xie, Cai-Xia
AU  - Jiang, Hong-Hua
AU  - Ding, Wen-Jun
AU  - Du, Peng
AU  - Chen, Wei
AU  - Yang, Ming
AU  - Cui, Long
TI  - Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer
DP  - 2012 Jan 01
TA  - Saudi Medical Journal
PG  - 24--29
VI  - 33
IP  - 1
4099  - http://smj.org.sa/content/33/1/24.short
4100  - http://smj.org.sa/content/33/1/24.full
SO  - Saudi Med J2012 Jan 01; 33
AB  - OBJECTIVE: To detect 4 MicroRNA (miRNA) in the stool samples of colorectal cancer (CRC) patients to determine whether these miRNAs could be biomarkers in CRC screening or treatment.METHODS: A retrospective comparison study was carried out in the Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China from September 2009 to March 2011. We detected 4 miRNAs (miR-143, miR-145, miR-21, and miR-106a) in the stool samples of 38 CRC patients and 13 healthy individuals. Total RNA from the stool samples was extracted using the EZNA TM stool RNA kit R6828-01. The miRNA quantification was carried out using TaqMan miRNA assays and the TaqMan Gene Expression Master Mix.RESULTS: The expression levels of miR-143 and miR-145 in the stool of the CRC patients were lower than in those of the healthy persons (p<0.005, median of 2-∆∆ct ). No statistically significant difference was found in the expression levels of both miR-21 and miR-106a between the stool of CRC patients and those of the healthy persons (p>0.05).CONCLUSION: The detection of fecal miRNAs is a potential method for CRC diagnosis or screening. Particularly, the down-regulation of fecal miR-143 and miR-145 could be a potential marker for CRC.