RT Journal Article
SR Electronic
T1 Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 24
OP 29
VO 33
IS 1
A1 Li, Jin-Ming
A1 Zhao, Rong-Hua
A1 Li, Shao-Tang
A1 Xie, Cai-Xia
A1 Jiang, Hong-Hua
A1 Ding, Wen-Jun
A1 Du, Peng
A1 Chen, Wei
A1 Yang, Ming
A1 Cui, Long
YR 2012
UL http://smj.org.sa/content/33/1/24.abstract
AB OBJECTIVE: To detect 4 MicroRNA (miRNA) in the stool samples of colorectal cancer (CRC) patients to determine whether these miRNAs could be biomarkers in CRC screening or treatment.METHODS: A retrospective comparison study was carried out in the Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China from September 2009 to March 2011. We detected 4 miRNAs (miR-143, miR-145, miR-21, and miR-106a) in the stool samples of 38 CRC patients and 13 healthy individuals. Total RNA from the stool samples was extracted using the EZNA TM stool RNA kit R6828-01. The miRNA quantification was carried out using TaqMan miRNA assays and the TaqMan Gene Expression Master Mix.RESULTS: The expression levels of miR-143 and miR-145 in the stool of the CRC patients were lower than in those of the healthy persons (p<0.005, median of 2-∆∆ct ). No statistically significant difference was found in the expression levels of both miR-21 and miR-106a between the stool of CRC patients and those of the healthy persons (p>0.05).CONCLUSION: The detection of fecal miRNAs is a potential method for CRC diagnosis or screening. Particularly, the down-regulation of fecal miR-143 and miR-145 could be a potential marker for CRC.