PT  - JOURNAL ARTICLE
AU  - Al-Sebayel, Mohammed I.
AU  - El-Sheikh, Yasser M.
AU  - Al-Mohanna, Falah H.
AU  - Al Abbad, Saleh I.
AU  - Al Nemry, Yaser H.
AU  - Al-Jammali, Ahmed
AU  - Al-Zeer, Norah K.
AU  - Alsebayel, Yazeed M.
AU  - Al-Bahli, Hamad M.
TI  - The use of ex-vivo liver perfusion circuit in sheep model
AID  - 10.15537/smj.2021.42.12.20210054
DP  - 2021 Dec 01
TA  - Saudi Medical Journal
PG  - 1289--1295
VI  - 42
IP  - 12
4099  - http://smj.org.sa/content/42/12/1289.short
4100  - http://smj.org.sa/content/42/12/1289.full
SO  - Saudi Med J2021 Dec 01; 42
AB  - Objectives: To describe a novel animal model for ex-vivo liver perfusion.Methods: This study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep.Results: Ex-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver.Conclusion: A normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible.