RT Journal Article
SR Electronic
T1 The use of ex-vivo liver perfusion circuit in sheep model
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1289
OP 1295
DO 10.15537/smj.2021.42.12.20210054
VO 42
IS 12
A1 Mohammed I. Al-Sebayel
A1 Yasser M. El-Sheikh
A1 Falah H. Al-Mohanna
A1 Saleh I. Al Abbad
A1 Yaser H. Al Nemry
A1 Ahmed Al-Jammali
A1 Norah K. Al-Zeer
A1 Yazeed M. Alsebayel
A1 Hamad M. Al-Bahli
YR 2021
UL http://smj.org.sa/content/42/12/1289.abstract
AB Objectives: To describe a novel animal model for ex-vivo liver perfusion.Methods: This study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep.Results: Ex-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver.Conclusion: A normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible.