PT  - JOURNAL ARTICLE
AU  - Waleed M. Bawazir
TI  - Genotyping of Dombrock blood group system in blood donors from Saudi Arabia
AID  - 10.15537/smj.2022.43.3.20210680
DP  - 2022 Mar 01
TA  - Saudi Medical Journal
PG  - 244--251
VI  - 43
IP  - 3
4099  - http://smj.org.sa/content/43/3/244.short
4100  - http://smj.org.sa/content/43/3/244.full
SO  - Saudi Med J2022 Mar 01; 43
AB  - Objectives: To establish the frequency of Dombrock (DO) blood group genotypes in Western Saudi Arabians and to compare the findings with other populations in the 1000 genomes database.Methods: This cross-sectional study was carried out between December 2018 and February 2019. A total of 440 blood samples in ethylenediaminetetraacetic acid tubes were collected from unrelated Saudi Arabian blood donors from Jeddah, Saudi Arabia. Deoxyribonucleic acid was extracted, followed by an allele-specific polymerase chain reaction for DO*01 and DO*02 alleles (c.793A>G, rs11276). The allele and genotype frequencies were counted and compared to those in other populations using the Chi-squared test with Bonferroni adjustments.Results: The DO allele frequencies for blood donors from western Saudi Arabia were 0.432 for DO*01 and 0.568 for DO*02. The DO genotype frequencies were 0.182 for DO*01/01, 0.318 for DO*02/02, and 0.5 for DO*01/02. The DO genotype frequencies were similar to Europeans, Americans, and South Asians but significantly different from the genotype frequencies of Africans and East Asians reported in the 1000 genomes database.Conclusion: Dombrock genotype frequencies in the Western Saudi Arabian population were different from Africans and East Asians but not from Europeans, Americans, and South Asians. This study contributes to a genotyped blood donor database and may advance transfusion safety for patients in western Saudi Arabia.