RT Journal Article
SR Electronic
T1 Phenotypic and genotypic detection of carbapenemase production among gram negative bacteria isolated from hospital acquired infections
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 236
OP 243
DO 10.15537/smj.2022.43.3.20210809
VO 43
IS 3
A1 Vamsi, Sreeja K.
A1 Moorthy, Rama S.
A1 Hemiliamma, Mary N.
A1 Chandra Reddy, Rama B.
A1 chanderakant, Deepak J.
A1 Sirikonda, Shravani
YR 2022
UL http://smj.org.sa/content/43/3/236.abstract
AB Objectives: To identify the carbapenemase producing Gram-negative bacteria (GNB) by phenotypic methods and to confirm the presence of resistant genes using real-time polymerase chain reaction (PCR).Methods: This was a prospective study carried out at the Department of Microbiology, Sri Venkata Sai Medical College and Hospital, Mahabubnagar, India, from March 2018-2021. All samples were screened for carbapenem resistance by disc diffusion method and the VITEK®2 compact system (bioMérieux, France). Detection of carbapenemase was carried out using RAPIDEC®CARBA NP test (Biomeriux Private Limited, South Delhi, India), screening for metallo-β-lactamases (MBL) was carried out by double disk synergy test (DDST), and genotypic characterization by real-time PCR.Results: Among the 1093 Gram-negative bacilli identified, 220 (17.0%) were resistant to carbapenems by both tested methods. Carbapenemase detection using the RAPIDEC®CARBA NP test indicated that 207 (94.0%) were carbapenemase producers, of which 189 (91.2%) were MBL producers. The most common carbapenemase genes identified were New Delhi metallo-β-lactamase (NDM; 47.3%), followed by the co-existence of genes in combination of NDM, with Verona integron-mediated metallo-β-lactamase (VIM; 39.6%), VIM and oxacillin hydrolyzing enzymes-48 (OXA-48; 4.3%), and OXA-48 (1.4%).No gene of active on imipenem, Klebsiella pneumonia carbapenemase, VIM, or OXA-48 alone was detected.Conclusion: This study suggests routine carbapenem resistance testing among multi-drug resistant-GNBs, as most of these infections occur in hospitals. In addition, there is a possibility that these highly antibiotic-resistant genes could spread to other bacteria resulting in further dissemination.