RT Journal Article
SR Electronic
T1 Nicotine and cotinine quantification after a 4-week inhalation of electronic cigarette vapors in male and female mice using UPLC-MS/MS
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 678
OP 686
DO 10.15537/smj.2022.43.7.20220142
VO 43
IS 7
A1 Fawaz Alasmari
A1 Abdullah F. Alasmari
A1 Ehab Elzayat
A1 Majed M. Alotaibi
A1 Farraj M. Alotaibi
A1 Mohamed W. Attwa
A1 Fars K. Alanazi
A1 Elkhatim H. Abdelgadir
A1 Syed Rizwan Ahmad
A1 Faleh Alqahtani
A1 Salim S. AL-Rejaie
A1 Musaad A. Alshammari
YR 2022
UL http://smj.org.sa/content/43/7/678.abstract
AB Objectives: To detect the cotinine and nicotine serum concentrations of female and male C57BL/6J mice after a 4-week exposure to electronic (e)-cigarette vapors using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods: This experimental study was carried out at an animal facility and laboratories, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia, between January and August 2020. A 4-week exposure to e-cigarettes was carried out using male and female mice and serum samples were obtained for cotinine and nicotine quantification using UPLC-MS/MS. The chromatographic procedures involved the use of a BEH HSS T3 C18 column (100 mm x 2.1 mm, 1.7 μm) with acetonitrile as a mobile phase and 0.1% formic acid (2:98 v/v).Results: The applied methodology has highly efficient properties of detection, estimation, and extraction, where the limit of quantification (LOQ) for nicotine was 0.57 ng/mL and limit of detection (LOD) for nicotine was 0.19 ng/mL, while the LOQ for cotinine was 1.11 ng/mL and LOD for cotinine was 0.38 ng/mL. The correlation coefficient was r2>0.99 for both compounds. The average recovery rate was 101.6±1.33 for nicotine and 100.4±0.54 for cotinine, while the precision and accuracy for cotinine and nicotine were less than 6.1. The serum cotinine level was higher in males (433.7±19.55) than females (362.3±16.27).Conclusion: This study showed that the gender factor might play a crucial role in nicotine metabolism.