RT Journal Article
SR Electronic
T1 Antibacterial and anti-biofilm activity of plumbagin against multi-drug resistant clinical bacterial isolates
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1224
OP 1233
DO 10.15537/smj.2022.43.11.20220446
VO 43
IS 11
A1 Alfhili, Mohammad A.
A1 Ahmad, Irfan
A1 Alraey, Yasser
A1 Alangari, Abdulaziz
A1 Alqahtani, Taha
A1 Dera, Ayed A.
YR 2022
UL http://smj.org.sa/content/43/11/1224.abstract
AB Objectives: To evaluate the antibacterial activity of plumbagin (PGN) against multidrug resistance (MDR) clinical isolates.Methods: This study was carried out at the Department of Clinical Lab Sciences, King Khalid University from October 6, 2021 to December 14, 2021. We investigated the antibacterial and anti-virulence activity of PGN against MDR Gram-negative (Escherichia coli, Klebsiella pneumoniae, Salmonella Typhi, and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus [S. aureus], Staphylococcus saprophyticus [S. saprophyticus], Streptococcus pyogenes, and Enterococcus faecalis) clinical bacterial isolates. Agar well diffusion, microdilution assay, colony count method, biofilm formation, and time-kill kinetics were employed to probe the MIC, MBC, and anti-virulence activity of PGN.Results: Plumbagin inhibited the growth of all tested isolates, with S. saprophyticus exhibiting the highest sensitivity. MIC values ranged from 0.029 to 0.117 µg/mL whereas MBC ranged from 0.235 to 0.94 µg/mL, with 79% to 99% growth inhibition. Moreover, all tested isolates showed a marked decrease in biofilm formation, with S. saprophyticus and S. aureus being the most sensitive.Conclusion: Plumbagin is a stand-alone, broad spectrum antibacterial with promising potential against the rising threat of antimicrobial resistance.