RT Journal Article
SR Electronic
T1 Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 246
OP 252
DO 10.15537/smj.2023.44.3.20220768
VO 44
IS 3
A1 ALkharashi, Nouf A.
YR 2023
UL http://smj.org.sa/content/44/3/246.abstract
AB Objectives: To evaluate the anti-cancer effect of resveratrol on Michigan cancer foundation-7 (MCF-7) and hepatoblastoma cell line (HepG2) cells.Methods: The study was carried out at the Department of Botany and Microbiology, Prince Sattam bin Abdulaziz University, Al-kharj, Saudi Arabia, from August 2022 to October 2022. Different concentrations of resveratrol were added to the MCF-7 and HepG2 cell lines. Cell death and proliferation were measured with MTT and Trypan blue exclusion assays. Apoptosis markers were assessed by using a quantitative PCR assay (qPCR).Results: The resveratrol was shown to suppress the proliferation of MCF-7 and HepG2 cells at dose- and time-dependent. The cytotoxic effect of resveratrol was observed even at 100 μM after 24 hours. In comparison to untreated cells, resveratrol treatment reduced the viability of MCF-7 cells to roughly 57.5% with a half maximal inhibitory concentration (IC50) of 51.18 μM and HepG2 cells to 56.2% with an IC50 of 57.4 μM. Furthermore, in the tested cell lines, resveratrol was able to induce apoptosis mediated by elevated apoptosis markers.Conclusion: Resveratrol appears to be an excellent candidate agent in anticancer therapy in various human cancers.