RT Journal Article SR Electronic T1 Development of a candidate multi-epitope vaccine against Sphingobacterium spiritivorum JF Saudi Medical Journal JO Saudi Med J FD Prince Sultan Military Medical City SP 544 OP 559 DO 10.15537/smj.2023.44.6.20220733 VO 44 IS 6 A1 Alamri, Mubarak A. YR 2023 UL http://smj.org.sa/content/44/6/544.abstract AB Objectives: To develop a candidate vaccine aginst the Sphingobacterium spiritivorum.Methods: Since there is currently no vaccine against this pathogen, we employed in-silico methods to extensively explore the outer membrane toxin-producing proteins found specifically in S. spiritivorum to forecast a multi-epitope chimeric vaccine design. This computational study was conducted in Saudi Arabia in 2022 (study design: computational; ethical approval not applicable).Results: TThe vaccine peptide comprises multiple linear and conformational B-cell epitopes, which have the potential to elicit humoral immunity. Projected B-cell- derived T-cell epitopes for outer membrane proteins are present in the produced protein. The docking and molecular dynamic simulation results indicating that the chimeric vaccine had adequate binding stability with TLR-4. Following the immunological simulation, significant levels of immune cell expression were observed as immunoglobulin (Ig) M and IgG, IgM, IgM1, and IgM2, and independently IgG1 and IgG2.Conclusion: The developed vaccine candidate is suitable for further testing and can assist experimental vaccinologists in developing an effective vaccine against S. spiritivorum.