PT  - JOURNAL ARTICLE
AU  - Ljubović, Amela Dedeić
AU  - Granov, Ðana
AU  - Husić, Erna
AU  - Gačanović, Džemilja
AU  - Halković, Jasmina
AU  - lab.ing, Azra Čamdžić
AU  - Kotorić Keser, Šejla
AU  - Loga Zec, Svjetlana
TI  - Prevalence of extended-spectrum β-lactamase and carbapenem-resistant <em>Klebsiella pneumoniae</em> in clinical samples
AID  - 10.15537/smj.2023.44.8.20230237
DP  - 2023 Aug 01
TA  - Saudi Medical Journal
PG  - 801--807
VI  - 44
IP  - 8
4099  - http://smj.org.sa/content/44/8/801.short
4100  - http://smj.org.sa/content/44/8/801.full
SO  - Saudi Med J2023 Aug 01; 44
AB  - Objectives: To assess the prevalence of these resistant strains in the overall isolates of Klebsiella pneumoniae (K. pneumoniae) in hospital settings.Methods: This retrospective study was conducted from November 2020 to November 2021. The identification and antibiotic susceptibility testing were performed using standard laboratory methods according to the EUCAST standards. The detection of ESBL and carbapenemase production was performed using phenotypic methods such as E-test, combined-disk test with various inhibitors (ROSCO Diagnostica A/S), chromogenic medium for the detection of ESBL/carbapenemase-producing Enterobacteriaceae (CPE) isolates, and the VITEK 2 Compact system (BioMerieux).Results: 944 isolates of K. pneumoniae were detected in various clinical specimens. Among these, ESBL-producing strains were detected in 349/944 (37%), whereas carbapenem- resistant strains in 188/944 (20%) of the isolates. The remaining isolates (407/944 [43%]) belonged to the wild type. ESBL isolates were the most common in wound swabs (138 [39.5%]), whereas CRKP isolates in screening samples (110 [58.5%]). The majority of ESBL isolates were detected in surgical departments (105 [30.1%]), whereas CRKP isolates in adult intensive care unit departments (79 [42.%]).Conclusion: Our results show an increasing frequency of CRKP strains. This presents a significant issue in terms of infection prevention and control in hospital settings.