PT  - JOURNAL ARTICLE
AU  - Durmuşlar, Mustafa C.
AU  - Gülşen, Elif A.
AU  - Bayburt, Kemal A.
AU  - Ballı, Umut
AU  - Ocak, Mert
AU  - Fırat, Ayşegül
AU  - Zırh, Elham B.
AU  - Çelik, Hakan H.
TI  - The effect of injectable platelet-rich fibrin in combination with autogenous bone grafts on bone healing in an ovariectomized osteoporotic rat model with critical-sized defects
AID  - 10.15537/smj.2024.45.8.20240224
DP  - 2024 Aug 01
TA  - Saudi Medical Journal
PG  - 791--798
VI  - 45
IP  - 8
4099  - http://smj.org.sa/content/45/8/791.short
4100  - http://smj.org.sa/content/45/8/791.full
SO  - Saudi Med J2024 Aug 01; 45
AB  - Objectives: To assess the impact of autogenous graft materials, including autogenous bone graft (ABG) and injectable platelet-rich fibrin (I-PRF) clots, on bone defect regeneration in ovariectomized osteoporotic rats.Methods: Wistar rats (6-8 weeks old) were ovariectomized, and surgery began after 8 weeks. A 5-mm defect was created bilaterally in the parietal bones of 16 rats, which were divided into 4 groups. Group A (blank) had untreated defects; group B had defects filled with 0.1 ml of I-PRF, group C had defects filled with 0.1 ml of ABG, and group D had defects filled with a combination of 0.1 ml each of I-PRF and ABG. Four weeks post-surgery, the rats were euthanized. Bone regeneration was evaluated through histopathologic analysis and microcomputed tomography (micro-CT).Results: In ovariectomized rats treated with ABG or I-PRF, bone regeneration was enhanced, with increased periosteal activity, osteoblast count, and new bone volume, as determined histologically. The ABG+I-PRF group had the highest periosteal vascularity, but the difference compared to the ABG group was not statistically significant (p>0.05). Osteoblast numbers were significantly higher in the ABG+I-PRF group than in the blank group (p<0.05). Micro-CT showed the highest mean new bone volume ratio in the ABG+I-PRF group, followed by the ABG group.Conclusion: The combined use of ABG and I-PRF enhances bone formation in osteoporotic rats following ovariectomy.