RT Journal Article
SR Electronic
T1 The effect of injectable platelet-rich fibrin in combination with autogenous bone grafts on bone healing in an ovariectomized osteoporotic rat model with critical-sized defects
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 791
OP 798
DO 10.15537/smj.2024.45.8.20240224
VO 45
IS 8
A1 Durmuşlar, Mustafa C.
A1 Gülşen, Elif A.
A1 Bayburt, Kemal A.
A1 Ballı, Umut
A1 Ocak, Mert
A1 Fırat, Ayşegül
A1 Zırh, Elham B.
A1 Çelik, Hakan H.
YR 2024
UL http://smj.org.sa/content/45/8/791.abstract
AB Objectives: To assess the impact of autogenous graft materials, including autogenous bone graft (ABG) and injectable platelet-rich fibrin (I-PRF) clots, on bone defect regeneration in ovariectomized osteoporotic rats.Methods: Wistar rats (6-8 weeks old) were ovariectomized, and surgery began after 8 weeks. A 5-mm defect was created bilaterally in the parietal bones of 16 rats, which were divided into 4 groups. Group A (blank) had untreated defects; group B had defects filled with 0.1 ml of I-PRF, group C had defects filled with 0.1 ml of ABG, and group D had defects filled with a combination of 0.1 ml each of I-PRF and ABG. Four weeks post-surgery, the rats were euthanized. Bone regeneration was evaluated through histopathologic analysis and microcomputed tomography (micro-CT).Results: In ovariectomized rats treated with ABG or I-PRF, bone regeneration was enhanced, with increased periosteal activity, osteoblast count, and new bone volume, as determined histologically. The ABG+I-PRF group had the highest periosteal vascularity, but the difference compared to the ABG group was not statistically significant (p>0.05). Osteoblast numbers were significantly higher in the ABG+I-PRF group than in the blank group (p<0.05). Micro-CT showed the highest mean new bone volume ratio in the ABG+I-PRF group, followed by the ABG group.Conclusion: The combined use of ABG and I-PRF enhances bone formation in osteoporotic rats following ovariectomy.