RT Journal Article
SR Electronic
T1 BOLA family genes are the drivers and potential biomarkers of survival in kidney renal clear cell carcinoma patients
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1207
OP 1218
DO 10.15537/smj.2024.45.11.20240604
VO 45
IS 11
A1 Alissa, Mohammed
A1 Alghamdi, Abdullah
A1 Alghamdi, Suad A.
A1 Alshehri, Mohammed A.
A1 Alsuwat, Meshari A.
A1 Allahyani, Mamdouh
A1 Alkhathami, Ali G.
YR 2024
UL http://smj.org.sa/content/45/11/1207.abstract
AB Objectives: To analyze the diagnostic and prognostic potential of the BOLA gene family in kidney renal clear cell carcinoma (KIRC).Methods: This study is an observational study. The whole study was carried out at Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia from January to November 2023. As it involves in silico and in vitro methods, ethical consent was not required. Various in silico and molecular experimental techniques were employed in this study.Results: Significant up-regulation and hypomethylation of BOLA1, BOLA2, and BOLA3 were observed across 12 KIRC cell lines. Retrospective analysis of the cancer genome atlas program (TCGA)-KIRC cohorts confirmed hypomethylation of these genes in KIRC tissues. The cBioPortal analysis showed minimal genetic alterations, with amplification being the most common. Kaplan-Meier plotter data revealed that high BOLA1, BOLA2, and BOLA3 expression correlated with shorter overall survival and relapse-free survival in KIRC. Tumor-immune system interactions database analysis linked BOLA1 expression to immune and molecular subtypes. Gene silencing of BOLA1, BOLA2, and BOLA3 in 786-0 cells reduced cell growth and proliferation, enhancing wound healing capacity.Conclusion: BOLA genes may serve as diagnostic and prognostic markers in KIRC, offering insights into therapeutic targets and disease progression.