RT Journal Article
SR Electronic
T1 Micro-RNA-21 rs1292037 A>G polymorphism can predict hepatocellular carcinoma prognosis (HCC), and plays a key role in cell proliferation and ischemia-reperfusion injury (IRI) in HCC cell model of IRI
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 383
OP 392
DO 10.15537/smj.2020.4.24994
VO 41
IS 4
A1 Wu, Chaoyu
A1 Tang, Gongen
A1 Wang, Xiaoying
A1 Zhang, Jian
A1 Chen, Shuhua
A1 Lu, Cuihong
A1 Zhang, Dongqin
A1 Li, Yun
YR 2020
UL http://smj.org.sa/content/41/4/383.abstract
AB Objectives: To assess the predictive function of miR-21 rs1292037 A>G polymorphism in survival and ischemia-reperfusion injury (IRI) in hepatocellular carcinoma (HCC) patients.Methods: The experiment was carried out between July 2018 and July 2019, whereas the prospective clinical study was carried out between January 2014 and January 2019. In this study, 62 HCC patients with hepatitis B virus (HBV), 17 HCC patients without HBV, and 13 healthy controls were investigated. Micro-RNA-21 levels in the blood were identified using quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and allele specific PCR for miR-21 rs1292037. Partial hepatectomy was performed in all patients, and the tumor development indicators and survival time were evaluated in a subsequent follow-up. Cell-based simulated IRI model of HCC huh7 cell line was used to determine the functions of miR-21 with respect to IRI and proliferation.Results: Micro-RNA-21 levels were significantly increased in HCC patients. Furthermore, an miR-21 rs1292037 A>G polymorphism was found to be significantly associated with HCC prognosis and severity of liver injury after hepatectomy. In vitro study revealed that miR-21 might prevent IRI from occurring, and can induce proliferation in HCC huh7 cells. These functions of miR-21 were demonstrated to be triggered by IL-12A inhibition.Conclusion: Micro-RNA-21 rs1292037 A>G polymorphism can predict IRI and tumor progression by regulating the miR-21/IL-12A.axis.