PT  - JOURNAL ARTICLE
AU  - Yunus E. Beyhan
AU  - Mehmet Karakus
AU  - Alper Karagoz
AU  - Mesut Mungan
AU  - Aysegul T. Ozkan
AU  - Murat Hokelek
TI  - Detection and identification of cutaneous leishmaniasis isolates by culture, polymerase chain reaction and sequence analyses in Syrian and Central Anatolia patients
AID  - 10.15537/smj.2017.9.21025
DP  - 2017 Aug 01
TA  - Saudi Medical Journal
PG  - 968--971
VI  - 38
IP  - 9
4099  - http://smj.org.sa/content/38/9/968.short
4100  - http://smj.org.sa/content/38/9/968.full
SO  - Saudi Med J2017 Aug 01; 38
AB  - Objectives: To characterize the cutaneous leishmaniasis (CL) isolates of Syrian and Central Anatolia patients at species levels.Methods: Skin scrapings of 3 patients (2 Syrian, 1 Turkish) were taken and examined by direct examination, culture in Novy-MacNeal-Nicole (NNN) medium, internal transcribed spacer polymerase chain reaction and sequence analysis (PCR).Results: According to microscopic examination, culture and PCR methods, 3 samples were detected positive. The sequencing results of all isolates in the study were identified as Leishmania tropica. The same genotypes were detected in the 3 isolates and nucleotide sequence submitted into GenBank with the accession number: KP689599.Conclusion: This finding could give information about the transmission of CL between Turkey and Syria. Because of the Syrian civil war, most of the Syrian citizens circulating in Turkey and different part of Europe, this can be increase the risk of spreading the disease. So, prevention measurements must be taken urgently.