PT  - JOURNAL ARTICLE
AU  - Ghani, Muhammad U.
AU  - Sabar, Muhammad F.
AU  - Bano, Iqbal
AU  - Shahid, Mariam
AU  - Akram, Muhammad
AU  - Khalid, Ifrah
AU  - Maryam, Alishba
AU  - Khan, Muhammad U.
TI  - Evaluation of <em>ADAM33</em> gene’s single nucleotide polymorphism variants against asthma and the unique pattern of inheritance in Northern and Central Punjab, Pakistan
AID  - 10.15537/smj.2019.8.24411
DP  - 2019 Aug 01
TA  - Saudi Medical Journal
PG  - 774--780
VI  - 40
IP  - 8
4099  - http://smj.org.sa/content/40/8/774.short
4100  - http://smj.org.sa/content/40/8/774.full
SO  - Saudi Med J2019 Aug 01; 40
AB  - Objectives: To investigate the relationship of 3 single nucleotide polymorphism (SNP) variants of ADAM33 with asthma susceptibility in patients from Northern and Central Punjab, Punjab, Pakistan.Methods: In this case-control study, healthy and asthmatic participants were recruited between 2015 and 2017. The SNPs of ADAM33 gene, rs2280089, rs2280090, and rs2280091 were analyzed in 296 asthma patients and 343 healthy controls, as well as linkage disequilibrium and haplotype analysis.Results: The non-significant differences were observed in allele and genotype frequencies of the SNPs in asthmatic and healthy persons even after population stratification based on age, caste, gender, family history, and environment. Although these SNPs were non-significant for disease susceptibility among children and adults, a fixed unique pattern of inheritance was nevertheless observed for the studied SNPs. Linkage disequilibrium analysis presented a very strong linkage between the SNP variants to predict their co-inheritance in study population. However, none of the haplotypes were found to be associated with asthma disease development.Conclusion: The studied SNPs of ADAM33 appeared to be non-significant for asthma susceptibility in Northern and Central Punjabi population. The fixed allele combination inheritance pattern was a unique observation contrary to findings in other global populations.