PT  - JOURNAL ARTICLE
AU  - Raddaoui, Emad M.
AU  - Almadi, Majid A.
AU  - Aljebreen, Abdulrahman M.
AU  - Alsaif, Faisal A.
AU  - AlShedoukhy, Ahlam A.
AU  - Al-Lehibi, Abed H.
AU  - Almohameed, Khalid A.
AU  - Tsolakis, Apostolos V.
AU  - AlAbbadi, Mousa A.
AU  - Almutrafi, Amna R.
TI  - Differential diagnosis between pancreatic neuroendocrine and solid pseudopapillary neoplasms on endoscopic ultrasound-guided fine-needle aspiration
AID  - 10.15537/smj.2016.7.14212
DP  - 2016 Jul 01
TA  - Saudi Medical Journal
PG  - 744--749
VI  - 37
IP  - 7
4099  - http://smj.org.sa/content/37/7/744.short
4100  - http://smj.org.sa/content/37/7/744.full
SO  - Saudi Med J2016 Jul 01; 37
AB  - Objectives: To evaluate the role of applying a limited panel of immunohistochemical stains on the cellblock preparation from samples obtained by endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) in the aim of differentiating solid pseudopapillary neoplasms (SPNs) from neuroendocrine tumors (NETs).Methods: We retrospectively retrieved all the EUS-FNAs of the pancreas that have a diagnosis of NET or SPN that were performed at 2 tertiary care hospitals in Riyadh, Kingdom of Saudi Arabia from May 2004 to December 2014. Diff-Quik, Papanicolaou, and Immunohistochemistry stains on cellblock preparations were performed.Results: Twenty cases were available (16 pancreatic neuroendocrine tumors (pNETs) and 4 SPNs). The pNETs were immunoreactive for synaptophysin, chromogranin A and CD56 while E-cadherin was diffusely to focally cytoplasmic positive. β-catenin was negative or showed focal cytoplasmic immunoreactivity. In comparison, SPNs were positive for vimentin, CD10, CD-56, focally positive for progesterone receptors and synaptophysin, and revealed nuclear immunostaining for β-catenin. They were negative for chromogranin A and E-cadherin.Conclusion: Based on EUS-FNA samples, nuclear immunoreactivity for β-catenin with loss of membranous immunostaining for E-Cadherin can potentially facilitate differentiating SPNs from pNETs.