RT Journal Article
SR Electronic
T1 Platelet-rich plasma can release nutrient factors to promote facial nerve crush injury recovery in rats
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1209
OP 1217
DO 10.15537/smj.2019.12.24747
VO 40
IS 12
A1 Li, Liheng
A1 Cai, Jing
A1 Yuan, Yang
A1 Mao, Yanyan
A1 Xu, Lei
A1 Han, Yuechen
A1 Li, Jianfeng
A1 Wang, Haibo
YR 2019
UL http://smj.org.sa/content/40/12/1209.abstract
AB Objectives: To evaluate the effects of platelet-rich plasma (PRP) on promoting neural repair after facial nerve compression in rats and the mechanism by which this occurs.Methods: Adult Wistar rats (n=100) were divided into 3 groups: healthy controls, surgery-only, and surgery+PRP groups. The rats underwent nerve crush injury to establish a facial palsy model. The blood from the rats was used to prepare the PRP for application to the injury site. The evaluation methods included vibrissae movement, eyelid closure, and electrophysiology. Electron microscopy, immunohistochemistry, and real-time polymerase chain reaction (PCR) were used to detect nutrient factor expression in the brain and nerve sections. This study was conducted in Shandong Provincial ENT Hospital Affiliated to Shandong University, Shandong, China between January and November 2018.Results: Platelet-rich plasma promotes the recovery of vibrissae movement, eyelid closure, and electrophysiological function in a rat model of nerve crush injury. Hematoxylin and eosin staining, toluidine blue staining, and electron microscopy showed significant recovery of Schwann cells and axons in the PRP group. Polymerase chain reaction results showed that PRP releases growth factors, which include nerve growth factor and brain-derived neurotrophic factor. Immunohistochemistry also demonstrated higher levels of S-100 protein expression in the PRP group compared to the other groups.Conclusions: Platelet-rich plasma releases nutrient factors in the brainstem, and the use of PRP can promote injury recovery.