RT Journal Article
SR Electronic
T1 HIV-TAT mediated protein transduction of heme oxygenase-1 protects HaCaT cells from ionizing radiation
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 234
OP 241
VO 35
IS 3
A1 Liu, Pengfei
A1 Xue, Jiao
A1 Gu, Qing
A1 Wu, Jinyong
A1 Cao, Han
A1 Zhu, Wei
A1 Zhou, Jundong
A1 Cao, Jianping
A1 Zhang, Shuyu
YR 2014
UL http://smj.org.sa/content/35/3/234.abstract
AB OBJECTIVE: To elucidate the effects of human keratinocyte-derived HaCaT cells (HIV-TAT) protein transduction domains (PTD) coupled heme oxygenase-1 (HO-1) fusion protein (TAT-HO-1) on radiation-induced human keratinocyte-derived HaCaT cells.METHODS: This study was conducted between May 2010 and February 2013 in the School of Radiation Medicine and Protection, Soochow University, Suzhou, China. This experimental study was designed to explore the protective role of TAT-HO-1 in skin cells. The human HO-1 gene was fused with a gene fragment encoding TAT PTD to produce in-frame TAT-HO-1. The distribution of TAT-HO-1 was measured by immunostaining and Western blot. The radioprotection for TAT-HO-1 was determined using clonogenic assay. Alterations in apoptosis were analyzed by flow cytometry.RESULTS: The expressed and purified TAT-HO-1 recombinant protein could be incorporated into human HaCaT cells. We then evaluated the protective role of TAT-HO-1 against ionizing radiation. The TAT-HO-1 attenuated the generation of reactive oxygen species and decreased HaCaT cell radiosensitivity to irradiation. Moreover, HaCaT cells pretreated with TAT-HO-1 resulted in less apoptosis by radiation. In addition, TAT-HO-1 could penetrate rat skin.CONCLUSION: These results suggest that TAT-HO-1 can protect HaCaT cells from ionizing radiation.