RT Journal Article
SR Electronic
T1 Correlation between expression of miR-155 in colon cancer and serum carcinoembryonic antigen level and its contribution to recurrence and metastasis forecast
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 547
OP 553
VO 35
IS 6
A1 Hongliang, Cao
A1 Shaojun, Huang
A1 Aihua, Liu
A1 Hua, Jiang
YR 2014
UL http://smj.org.sa/content/35/6/547.abstract
AB OBJECTIVE: To analyze the correlation between expression of miR-155 in colon cancer tissue and serum carcinoembryonic antigen (CEA) levels, and then explore its contribution to forecasting recurrence and metastasis.METHODS: Eighty-four pairs of colon cancer specimens and their corresponding non-tumor adjacent tissues were collected and analyzed between March 2009 and December 2011 in Xiangyang Central Hospital, Xiangyang, Hubei, China. The expression of miR-155 in both tissues was tested using reverse transcription-polymerase chain reaction (RT-PCR), the preoperative serum CEA level was assessed, and the postoperative serum CEA level was also assessed bimonthly during the follow-up period of 2 years.RESULTS: The expression of miR-155 in colon cancer tissue was significantly higher than that in normal tissues (p<0.05), it had an obvious positive correlation with the preoperative serum CEA levels (p<0.01), and a negative correlation with time of duration since the serum CEA level increased again postoperatively (p<0.01). The expression of miR-155 in the recurrence and metastasis group was significantly higher (6.06+/-3.73 times) than that in the non-recurrence and non-metastasis group (p<0.05). An increase in the postoperative serum CEA levels was significantly correlated with recurrence and metastasis of the tumor postoperatively.CONCLUSION: The expression of miR-155 is up-regulated in colon cancer tissue. A combination of miR-155 level assay in colon cancer tissue and the serum CEA level both pre- and postoperatively can afford more accurate information for diagnosis and prognosis, especially for predicting recurrence and metastasis postoperatively.