PT  - JOURNAL ARTICLE
AU  - Khalil A. Aziz
TI  - Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils
DP  - 2005 Oct 01
TA  - Saudi Medical Journal
PG  - 1596--1601
VI  - 26
IP  - 10
4099  - http://smj.org.sa/content/26/10/1596.short
4100  - http://smj.org.sa/content/26/10/1596.full
SO  - Saudi Med J2005 Oct 01; 26
AB  - OBJECTIVE: To investigate the mechanism of CD11b down-regulation in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN).METHODS: Purified PMN were stimulated with PMA in the presence, or absence, of various enzyme inhibitors. Following stimulation, PMN CD11b expression was examined by flow cytometry and Western-blotting. The entire work was carried out at Liverpool University between the period of 1998 and 2001.RESULTS: Stimulation of PMN with PMA induced the down-regulation of CD11b by a mechanism involving a combination of an oxidant and a serine protease; most likely the primary granule derived elastase.CONCLUSION: The present study shows for the first time the cooperation of both oxidants and enzymes in the down-regulation of PMN adhesion receptors.