RT Journal Article
SR Electronic
T1 Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils
JF Saudi Medical Journal
JO Saudi Med J
FD Prince Sultan Military Medical City
SP 1596
OP 1601
VO 26
IS 10
A1 Khalil A. Aziz
YR 2005
UL http://smj.org.sa/content/26/10/1596.abstract
AB OBJECTIVE: To investigate the mechanism of CD11b down-regulation in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN).METHODS: Purified PMN were stimulated with PMA in the presence, or absence, of various enzyme inhibitors. Following stimulation, PMN CD11b expression was examined by flow cytometry and Western-blotting. The entire work was carried out at Liverpool University between the period of 1998 and 2001.RESULTS: Stimulation of PMN with PMA induced the down-regulation of CD11b by a mechanism involving a combination of an oxidant and a serine protease; most likely the primary granule derived elastase.CONCLUSION: The present study shows for the first time the cooperation of both oxidants and enzymes in the down-regulation of PMN adhesion receptors.