PT  - JOURNAL ARTICLE
AU  - Hassanshahi, Gholamhossein
AU  - Patel, Sanjai S.
AU  - Jafarzadeh, Abdollah A.
AU  - Dickson, Alan J.
TI  - Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock
DP  - 2007 Apr 01
TA  - Saudi Medical Journal
PG  - 514--518
VI  - 28
IP  - 4
4099  - http://smj.org.sa/content/28/4/514.short
4100  - http://smj.org.sa/content/28/4/514.full
SO  - Saudi Med J2007 Apr 01; 28
AB  - OBJECTIVE: To examine the expression of chemokine IP-10/Mob-1 of hepatocytes in responses to the stress imposed during isolation by collagenase perfusion.METHODS: This study was performed in the Faculty of Life Sciences University of Manchester during 2001-2005. We employed western and northern blotting analysis to detect IP-10/Mob-1 in isolated and cultured hepatocytes in response to isolation stresses and under heat shock stimulation in this project.RESULTS: We showed that the ELR-CXC chemokine, IP-10/Mob-1 is secreted from isolated rat hepatocytes immediately after isolation and early during culture and IP-10/Mob-1, expression by hepatocytes was also stimulated in response to heat shock.CONCLUSION: It seems that hepatocytes mimic the experiences of liver injury in vivo such as during stress, trauma, or after insults, and therefore, produce stress related agents like IP-10/Mob-1 chemokine to overcome such a injurious condition following isolation and heat shock stimulation. This study also provides a useful model to study the regulation of expression of this chemokines in vitro.